• Western blot analysis of lysates from COS7 cells treated with TNF 20ng/ml 5', using GRK1 (Phospho-Ser21) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain, using GRK1 (Phospho-Ser21) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-GRK1-Ser21 antibody (6-55 aa) (STJ90901)

SKU:
STJ90901

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Rhodopsin kinase GRK1-Ser21 (6-55 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRK1
Gene ID: 6011
Uniprot ID: GRK1_HUMAN
Immunogen Region: 6-55 aa
Specificity: Phospho-GRK 1 (S21) Polyclonal Antibody detects endogenous levels of GRK 1 protein only when phosphorylated at S21.
Immunogen: The antiserum was produced against synthesized peptide derived from the human GRK1 around the phosphorylation site of Ser21 at the amino acid range 6-55
Post Translational Modifications Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492. Phosphorylation at Ser-21 is regulated by light and activated by cAMP. Farnesylation is required for full activity.
Function Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination. May play a role in the maintenance of the outer nuclear layer in the retina.
Protein Name Rhodopsin Kinase Grk1
Rk
G Protein-Coupled Receptor Kinase 1
Database Links Reactome: R-HSA-2514859
Cellular Localisation Membrane
Lipid-Anchor
Cell Projection
Cilium
Photoreceptor Outer Segment
Subcellular Location Is Not Affected By Light Or Dark Conditions
Alternative Antibody Names Anti-Rhodopsin Kinase Grk1 antibody
Anti-Rk antibody
Anti-G Protein-Coupled Receptor Kinase 1 antibody
Anti-GRK1 antibody
Anti-RHOK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance