| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Tyrosine-protein kinase Fgr oncogene homolog-Tyr412 is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
FGR |
| Gene ID: |
2268 |
| Uniprot ID: |
FGR_HUMAN |
| Specificity: |
This antibody detects endogenous levels of FGR (Phospho-Tyr412) at Human, Mouse, Rat |
| Immunogen: |
Synthesized peptide derived from human FGR (Phospho-Tyr412) |
| Tissue Specificity | Detected in neutrophils, monocytes and natural killer cells (at protein level). Detected in monocytes and large lymphocytes. |
| Post Translational Modifications | Ubiquitinated. Becomes ubiquitinated in response to ITGB2 signaling.this does not lead to degradation. Phosphorylated. Autophosphorylated on tyrosine residues. Becomes phosphorylated in response to FCGR2A and/or FCGR2B engagement, cell adhesion and signaling by ITGB2. Prior phosphorylation at Tyr-523 by SRC inhibits ulterior autophosphorylation at Tyr-412. |
| Function | Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors devoid of kinase activity and contributes to the regulation of immune responses, including neutrophil, monocyte, macrophage and mast cell functions, cytoskeleton remodeling in response to extracellular stimuli, phagocytosis, cell adhesion and migration. Promotes mast cell degranulation, release of inflammatory cytokines and IgE-mediated anaphylaxis. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as MS4A2/FCER1B, FCGR2A and/or FCGR2B. Acts downstream of ITGB1 and ITGB2, and regulates actin cytoskeleton reorganization, cell spreading and adhesion. Depending on the context, activates or inhibits cellular responses. Functions as negative regulator of ITGB2 signaling, phagocytosis and SYK activity in monocytes. Required for normal ITGB1 and ITGB2 signaling, normal cell spreading and adhesion in neutrophils and macrophages. Functions as positive regulator of cell migration and regulates cytoskeleton reorganization via RAC1 activation. Phosphorylates SYK (in vitro) and promotes SYK-dependent activation of AKT1 and MAP kinase signaling. Phosphorylates PLD2 in antigen-stimulated mast cells, leading to PLD2 activation and the production of the signaling molecules lysophosphatidic acid and diacylglycerol. Promotes activation of PIK3R1. Phosphorylates FASLG, and thereby regulates its ubiquitination and subsequent internalization. Phosphorylates ABL1. Promotes phosphorylation of CBL, CTTN, PIK3R1, PTK2/FAK1, PTK2B/PYK2 and VAV2. Phosphorylates HCLS1 that has already been phosphorylated by SYK, but not unphosphorylated HCLS1. Together with CLNK, it acts as a negative regulator of natural killer cell-activating receptors and inhibits interferon-gamma production. |
| Protein Name | Tyrosine-Protein Kinase FgrGardner-Rasheed Feline Sarcoma ViralV-Fgr Oncogene HomologProto-Oncogene C-FgrP55-FgrP58-FgrP58c-Fgr |
| Database Links | Reactome: R-HSA-2029481Reactome: R-HSA-432142Reactome: R-HSA-6798695Reactome: R-HSA-9664323Reactome: R-HSA-9664422 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SidePeripheral Membrane ProteinCell ProjectionRuffle MembraneCytoplasmCytosolCytoskeletonMitochondrion Inner MembraneMitochondrion Intermembrane SpaceDetected In Mitochondrial Intermembrane Space And At Inner MembranesColocalizes With Actin Fibers At Membrane RufflesDetected At Plasma Membrane Lipid Rafts |
| Alternative Antibody Names | Anti-Tyrosine-Protein Kinase Fgr antibodyAnti-Gardner-Rasheed Feline Sarcoma Viral antibodyAnti-V-Fgr Oncogene Homolog antibodyAnti-Proto-Oncogene C-Fgr antibodyAnti-P55-Fgr antibodyAnti-P58-Fgr antibodyAnti-P58c-Fgr antibodyAnti-FGR antibodyAnti-SRC2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
