• Western Blot analysis of mouse spleen, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-FANCD2-Thr691 antibody (STJA0010051)

SKU:
STJA0010051

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Fanconi anemia group D2 protein-Thr691 is suitable for use in Immunohistochemistry and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FANCD2
Gene ID: 2177
Uniprot ID: FACD2_HUMAN
Specificity: This antibody detects endogenous levels of FANCD2 (Phospho Thr691) Rabbit pAb at Human, Mouse, Rat
Immunogen: Synthesized peptide derived from human FANCD2 (Phospho Thr691)
Tissue Specificity Highly expressed in germinal center cells of the spleen, tonsil, and reactive lymph nodes, and in the proliferating basal layer of squamous epithelium of tonsil, esophagus, oropharynx, larynx and cervix. Expressed in cytotrophoblastic cells of the placenta and exocrine cells of the pancreas (at protein level). Highly expressed in testis, where expression is restricted to maturing spermatocytes.
Post Translational Modifications Monoubiquitinated on Lys-561 during S phase and upon genotoxic stress by FANCL in complex with E2 ligases UBE2T or UBE2W (isoform 1 and isoform 2). Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the joint intervention of the FANC core complex, including FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, and FANCM, and proteins involved in cell cycle checkpoints and DNA repair, including RPA1, ATR, CHEK1 and BRCA1, and is mediated by FANCL/PHF9. Ubiquitination is required for binding to chromatin, interaction with BRCA1, BRCA2 and MTMR15/FAN1, DNA repair, and normal cell cycle progression, but not for phosphorylation on Ser-222 or interaction with MEN1. Phosphorylated in response to various genotoxic stresses by ATM and/or ATR. Upon ionizing radiation, phosphorylated by ATM on Ser-222 and Ser-1404. Phosphorylation on Ser-222 is required for S-phase checkpoint activation, but not for ubiquitination, foci formation, or DNA repair. In contrast, phosphorylation by ATR on other sites may be required for ubiquitination and foci formation.
Function Required for maintenance of chromosomal stability. Promotes accurate and efficient pairing of homologs during meiosis. Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing. May participate in S phase and G2 phase checkpoint activation upon DNA damage. Plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress. Required for the targeting, or stabilization, of BLM to non-centromeric abnormal structures induced by replicative stress. Promotes BRCA2/FANCD1 loading onto damaged chromatin. May also be involved in B-cell immunoglobulin isotype switching.
Protein Name Fanconi Anemia Group D2 Protein
Protein Facd2
Database Links Reactome: R-HSA-6783310
Reactome: R-HSA-6796648
Cellular Localisation Nucleus
Concentrates In Nuclear Foci During S Phase And Upon Genotoxic Stress
At The Onset Of Mitosis
Excluded From Chromosomes And Diffuses Into The Cytoplasm
Returning To The Nucleus At The End Of Cell Division
Observed In A Few Spots Localized In Pairs On The Sister Chromatids Of Mitotic Chromosome Arms And Not Centromeres
One On Each Chromatids
These Foci Coincide With Common Fragile Sites And Could Be Sites Of Replication Fork Stalling
The Foci Are Frequently Interlinked Through Blm-Associated Ultra-Fine Dna Bridges
Following Aphidicolin Treatment
Targets Chromatid Gaps And Breaks
Alternative Antibody Names Anti-Fanconi Anemia Group D2 Protein antibody
Anti-Protein Facd2 antibody
Anti-FANCD2 antibody
Anti-FACD antibody

Information sourced from Uniprot.org

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