Host: | Rabbit |
Applications: | WB/IHC/IF/ELISA |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-Phospho-Estrogen receptor-Ser104 (71-120 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration: | 1 mg/mL |
Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:5000 IF 1:50-200 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | ESR1 |
Gene ID: | 2099 |
Uniprot ID: | ESR1_HUMAN |
Immunogen Region: | 71-120 aa |
Specificity: | Phospho-ER Alpha (S104) Polyclonal Antibody detects endogenous levels of ER Alpha protein only when phosphorylated at S104. |
Immunogen: | The antiserum was produced against synthesized peptide derived from the human Estrogen Receptor-alpha around the phosphorylation site of Ser104 at the amino acid range 71-120 |
Post Translational Modifications | Phosphorylated by cyclin A/CDK2 and CK1. Phosphorylation probably enhances transcriptional activity. Self-association induces phosphorylation. Dephosphorylation at Ser-118 by PPP5C inhibits its transactivation activity. Phosphorylated by LMTK3 in vitro. Glycosylated.contains N-acetylglucosamine, probably O-linked. Ubiquitinated.regulated by LATS1 via DCAF1 it leads to ESR1 proteasomal degradation. Deubiquitinated by OTUB1. Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization. Demethylated by JMJD6 at Arg-260. Palmitoylated (isoform 3). Not biotinylated (isoform 3). Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation, but not for signaling mediated by the nuclear hormone receptor. |
Function | Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements.the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3: Involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor. Binds to ERE and inhibits isoform 1. |
Protein Name | Estrogen Receptor Er Er-Alpha Estradiol Receptor Nuclear Receptor Subfamily 3 Group A Member 1 |
Database Links | Reactome: R-HSA-1251985 Reactome: R-HSA-1257604 Reactome: R-HSA-2219530 Reactome: R-HSA-383280 Reactome: R-HSA-4090294 Reactome: R-HSA-5689896 Reactome: R-HSA-6811558 Reactome: R-HSA-8866910 Reactome: R-HSA-8931987 Reactome: R-HSA-8939211 Reactome: R-HSA-8939256 Reactome: R-HSA-8939902 Reactome: R-HSA-9009391 Reactome: R-HSA-9018519 |
Cellular Localisation | Isoform 1: Nucleus Cytoplasm Cell Membrane Peripheral Membrane Protein Cytoplasmic Side A Minor Fraction Is Associated With The Inner Membrane Isoform 3: Nucleus Single-Pass Type I Membrane Protein Associated With The Inner Membrane Via Palmitoylation (Probable) At Least A Subset Exists As A Transmembrane Protein With A N-Terminal Extracellular Domain Nucleus Golgi Apparatus Colocalizes With Zdhhc7 And Zdhhc21 In The Golgi Apparatus Where Most Probably Palmitoylation Occurs Associated With The Plasma Membrane When Palmitoylated |
Alternative Antibody Names | Anti-Estrogen Receptor antibody Anti-Er antibody Anti-Er-Alpha antibody Anti-Estradiol Receptor antibody Anti-Nuclear Receptor Subfamily 3 Group A Member 1 antibody Anti-ESR1 antibody Anti-ESR antibody Anti-NR3A1 antibody |
Information sourced from Uniprot.org