• Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 rabbit monoclonal antibody (STJ11102568) at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of Jurkat cells (treated with PMA) and Jurkat cells (untreated) , using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit monoclonal antibody (STJ11102568) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of C6 cells, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 rabbit monoclonal antibody (STJ11102568) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 in paraffin-embedded rat fallopian tube using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit monoclonal antibody (STJ11102568) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat brain using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 rabbit monoclonal antibody (STJ11102568) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 in paraffin-embedded rat brain using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit monoclonal antibody (STJ11102568) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat fallopian tube using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 rabbit monoclonal antibody (STJ11102568) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from C6 cells, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit monoclonal antibody (STJ11102568) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunofluorescence analysis of Jurkat cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 rabbit monoclonal antibody (STJ11102568) at dilution of 1:100 (40x lens). Jurkat cells were treated by PMA/TPA (200 nM) at 37 °C for 10 minutes. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit monoclonal antibody (STJ11102568) at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-MAPK1/MAPK3-T202/Y204 & T185/Y187 antibody [S8MR] (STJ11102568)

SKU:
STJ11102568

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Phospho-MAPK1/MAPK3-T202/Y204 & T185/Y187 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic phosphorylated peptide around T202 & Y204 of human ERK1 (P27361).
Immunogen Sequence: LTEYV
Background Acts upstream of or within cellular response to starvation. Located in myofibril. Orthologous to human MYH13 (myosin heavy chain 13).

Information sourced from Uniprot.org

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