• Western blot of recombinant Elk-1 showing specific immunolabeling of the ~46 kDa Elk-1 phosphorylated at Ser383 in the right lane (-). Phosphospecificity is shown in the left lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-ELK1-Ser383 antibody (STJA0003626)

SKU:
STJA0003626-100

£442.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat/Canine/Human/Non-Human Primates/Zebrafish
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Elk-1-Ser383 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ELK1
Gene ID: 2002
Uniprot ID: ELK1_HUMAN
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 conjugated to KLH.
Tissue Specificity Lung and testis.
Post Translational Modifications Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention. On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Upon bFGF stimulus, phosphorylated by PAK1.
Function Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation.
Protein Name Ets Domain-Containing Protein Elk-1
Database Links Reactome: R-HSA-198753
Reactome: R-HSA-9031628
Reactome: R-HSA-9609690
Reactome: R-HSA-9634638
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Ets Domain-Containing Protein Elk-1 antibody
Anti-ELK1 antibody

Information sourced from Uniprot.org

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