Anti-Phospho-EEF2K-Ser-445 antibody (STJA0005393)

SKU:
STJA0005393-100

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat/Xenopus
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-eEF2K-Ser-445 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Purification: Antigen Affinity Purified
Dilution Range: WB 1:1000
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EEF2K
Gene ID: 29904
Uniprot ID: EF2K_HUMAN
Specificity: The antibody was cross-absorbed to unphosphorylated eEF2K (Ser-445) before affinity purification using phospho-eEF2K (Ser-445) peptide (without carrier). This antibody detects a 105 kDa* protein corresponding to the molecular mass of eEF2K on SDS-PAG
Immunogen: Phospho-eEF2K (Ser-445) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-445 in human eEF2K. This sequence is well conserved in rat, mouse, and fish eEF2K, and has low homology to other proteins.
Post Translational Modifications Autophosphorylated at multiple residues, Thr-348 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-398 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 at Ser-366 instead decrease EEF2K activity and promote protein synthesis.
Function Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
Protein Name Eukaryotic Elongation Factor 2 Kinase
Eef-2 Kinase
Eef-2k
Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase
Database Links Reactome: R-HSA-166208
Alternative Antibody Names Anti-Eukaryotic Elongation Factor 2 Kinase antibody
Anti-Eef-2 Kinase antibody
Anti-Eef-2k antibody
Anti-Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase antibody
Anti-EEF2K antibody

Information sourced from Uniprot.org

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