Anti-Phospho-E2F1-Thr433 antibody (388-437 aa) (STJ90458)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human E2F1 around the phosphorylation site of Thr433 at the amino acid range 388-437
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Transcription factor E2F1-Thr433 (388-437 aa) for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:5000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | E2F1 |
| Gene ID | 1869 |
| Uniprot ID | E2F1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human E2F1 around the phosphorylation site of Thr433 at the amino acid range 388-437 |
| Immunogen Region | 388-437 aa |
| Specificity | Phospho-E2F-1 (T433) Polyclonal Antibody detects endogenous levels of E2F-1 protein only when phosphorylated at T433. |
Additional Info
| Function | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTCCGCGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters. Directly activates transcription of PEG10. Positively regulates transcription of RRP1B. |
| Protein Name | Transcription Factor E2f1E2f-1Pbr3Retinoblastoma-Associated Protein 1Rbap-1Retinoblastoma-Binding Protein 3Rbbp-3Prb-Binding Protein E2f-1 |
| Database Links | Reactome: R-HSA-111448Reactome: R-HSA-113501Reactome: R-HSA-1362277Reactome: R-HSA-1362300Reactome: R-HSA-139915Reactome: R-HSA-1912408Reactome: R-HSA-2559580Reactome: R-HSA-2559585Reactome: R-HSA-6804116Reactome: R-HSA-68911Reactome: R-HSA-69202Reactome: R-HSA-69205Reactome: R-HSA-69231Reactome: R-HSA-69656Reactome: R-HSA-8953750Reactome: R-HSA-9616222Reactome: R-HSA-9661069 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Factor E2f1 antibodyAnti-E2f-1 antibodyAnti-Pbr3 antibodyAnti-Retinoblastoma-Associated Protein 1 antibodyAnti-Rbap-1 antibodyAnti-Retinoblastoma-Binding Protein 3 antibodyAnti-Rbbp-3 antibodyAnti-Prb-Binding Protein E2f-1 antibodyAnti-E2F1 antibodyAnti-RBBP3 antibody |
Information sourced from Uniprot.org