Anti-Phospho-DOK2-Y299 antibody (STJ11105773)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11105773
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General Information

Short DescriptionRabbit polyclonal Phospho-Docking Protein 2-Y299 antibody for use in WB, IF and ICC in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IF/ICC:1:100-1:200
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolDOK2
Gene ID9046
Uniprot IDDOK2_HUMAN
SpecificityA phospho specific peptide corresponding to residues surrounding Y299 of human DOK2

Additional Info

Tissue Specificity Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.
Post Translational Modifications On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP. Phosphorylated on tyrosine residues by TEK/TIE2.
Function DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.
Protein Name Docking Protein 2
Downstream Of Tyrosine Kinase 2
P56(Dok-2
Database Links Reactome: R-HSA-210993
Reactome: R-HSA-8853659
Cellular Localisation
Alternative Antibody Names Anti-Docking Protein 2 antibody
Anti-Downstream Of Tyrosine Kinase 2 antibody
Anti-P56(Dok-2 antibody
Anti-DOK2 antibody

Information sourced from Uniprot.org

Citations

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