• Western blot analysis of lysates from COS7 cells treated with insulin 0.01U/ml 15', Jurkat cells treated with insulin 0.01U/ml 15' and 293 cells treated with serum 20% 15', using p56 Dok-2 (Phospho-Tyr299) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Dok-2 (Y299) Polyclonal Antibody

Anti-Phospho-DOK2-Tyr299 antibody (266-315 aa) (STJ90245)

SKU:
STJ90245

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IF/ELISA
Reactivity: Human/Mouse/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Docking protein 2-Tyr299 (266-315 aa) is suitable for use in Western Blot, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DOK2
Gene ID: 9046
Uniprot ID: DOK2_HUMAN
Immunogen Region: 266-315 aa
Specificity: Phospho-Dok-2 (Y299) Polyclonal Antibody detects endogenous levels of Dok-2 protein only when phosphorylated at Y299.
Immunogen: The antiserum was produced against synthesized peptide derived from the human p56 Dok-2 around the phosphorylation site of Tyr299 at the amino acid range 266-315
Post Translational Modifications On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP. Phosphorylated on tyrosine residues by TEK/TIE2.
Function DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.
Protein Name Docking Protein 2
Downstream Of Tyrosine Kinase 2
P56(Dok-2
Database Links Reactome: R-HSA-210993
Reactome: R-HSA-8853659
Alternative Antibody Names Anti-Docking Protein 2 antibody
Anti-Downstream Of Tyrosine Kinase 2 antibody
Anti-P56(Dok-2 antibody
Anti-DOK2 antibody

Information sourced from Uniprot.org

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