• Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95 kDa dynamin phosphorylated at Ser774 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-DNM1-Ser774 antibody (STJA0003622)

SKU:
STJA0003622-100

£442.50
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Host: Sheep
Applications: WB/ICC
Reactivity: Mouse/Rat/Bovine/Canine/Chicken/Human/Non-Human Primates
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Sheep polyclonal antibody anti-Phospho-Dynamin-Ser774 is suitable for use in Western Blot and Immunocytochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC
ICC 1:1000
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Dnm1
Gene ID: 140694
Uniprot ID: DYN1_RAT
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 conjugated to KLH.
Tissue Specificity Brain-specific (peripheral sensory neurons).
Post Translational Modifications Constitutively phosphorylated by CDK5 in nerve terminals and dephosphorylated by calcineurin when synaptic vesicle endocytosis (SVE) is stimulated by depolarization-dependent calcium influx.
Function Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis.
Protein Name Dynamin-1
B-Dynamin
D100
Dynamin - Brain
Database Links Reactome: R-RNO-190873
Reactome: -RNO-196025
Reactome: -RNO-2132295
Reactome: -RNO-437239
Reactome: -RNO-8856828
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule-Associated
Isoform-Specific Localization
Isoform 2: Cytoplasm
Isoform 6: Cytoplasm
Golgi Apparatus
Alternative Antibody Names Anti-Dynamin-1 antibody
Anti-B-Dynamin antibody
Anti-D100 antibody
Anti-Dynamin - Brain antibody
Anti-Dnm1 antibody
Anti-Dnm antibody

Information sourced from Uniprot.org

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