Anti-Phospho-DMTN-Ser403 antibody (356-405 aa) (STJ90897)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Dematin around the phosphorylation site of Ser403 at the amino acid range 356-405
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Dematin-Ser403 (356-405 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DMTN |
| Gene ID | 2039 |
| Uniprot ID | DEMA_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Dematin around the phosphorylation site of Ser403 at the amino acid range 356-405 |
| Immunogen Region | 356-405 aa |
| Specificity | Phospho-Dematin (S403) Polyclonal Antibody detects endogenous levels of Dematin protein only when phosphorylated at S403. |
Additional Info
| Post Translational Modifications | Phosphorylated. Phosphorylation at Ser-403 by PKA causes the C-terminal headpiece domain to associate with the N-terminal core domain, and leads to the inhibition of its actin bundling activity. The N-terminus is blocked. |
| Function | Membrane-cytoskeleton-associated protein with F-actin-binding activity that induces F-actin bundles formation and stabilization. Its F-actin-bundling activity is reversibly regulated upon its phosphorylation by the cAMP-dependent protein kinase A (PKA). Binds to the erythrocyte membrane glucose transporter-1 SLC2A1/GLUT1, and hence stabilizes and attaches the spectrin-actin network to the erythrocytic plasma membrane. Plays a role in maintaining the functional integrity of PKA-activated erythrocyte shape and the membrane mechanical properties. Also plays a role as a modulator of actin dynamics in fibroblasts.acts as a negative regulator of the RhoA activation pathway. In platelets, functions as a regulator of internal calcium mobilization across the dense tubular system that affects platelet granule secretion pathways and aggregation. Also required for the formation of a diverse set of cell protrusions, such as filopodia and lamellipodia, necessary for platelet cell spreading, motility and migration. Acts as a tumor suppressor and inhibits malignant cell transformation. |
| Protein Name | DematinDematin Actin-Binding ProteinErythrocyte Membrane Protein Band 4.9 |
| Database Links | Reactome: R-HSA-5223345 |
| Cellular Localisation | CytoplasmCytosolPerinuclear RegionCytoskeletonCell MembraneMembraneEndomembrane SystemCell ProjectionLocalized At The Spectrin-Actin Junction Of Erythrocyte Plasma MembraneLocalized To Intracellular Membranes And The Cytoskeletal NetworkLocalized At Intracellular Membrane-Bounded Organelle Compartment In Platelets That Likely Represent The Dense Tubular Network MembraneDetected At The Cell Membrane And At The Parasitophorous Vacuole In Malaria-Infected Erythrocytes At Late Stages Of Plasmodium Berghei Or Falciparum Development |
| Alternative Antibody Names | Anti-Dematin antibodyAnti-Dematin Actin-Binding Protein antibodyAnti-Erythrocyte Membrane Protein Band 4.9 antibodyAnti-DMTN antibodyAnti-DMT antibodyAnti-EPB49 antibody |
Information sourced from Uniprot.org