• Western blot of mouse nuclei lysate showing specific immunolabeling of the ~120 kDa DGCR8 protein phosphorylated at in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-DGCR8-Ser377 antibody (STJA0003611)

SKU:
STJA0003611-100

£442.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-DGCR8-Ser377 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DGCR8
Gene ID: 54487
Uniprot ID: DGCR8_HUMAN
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser377 conjugated to KLH.
Tissue Specificity Ubiquitously expressed.
Function Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing. Involved in the silencing of embryonic stem cell self-renewal.
Protein Name Microprocessor Complex Subunit Dgcr8
Digeorge Syndrome Critical Region 8
Database Links Reactome: R-HSA-203927
Reactome: R-HSA-8986944
Cellular Localisation Nucleus
Nucleolus
Colocalizes With Nucleolin And Drosha In The Nucleolus
Mostly Detected In The Nucleolus As Electron-Dense Granular Patches Around The Fibrillar Center (Fc) And Granular Component (Gc)
Also Detected In The Nucleoplasm As Small Foci Adjacent To Splicing Speckles Near The Chromatin Structure
Localized With Drosha In Gw Bodies (Gwbs)
Also Known As P-Bodies
Alternative Antibody Names Anti-Microprocessor Complex Subunit Dgcr8 antibody
Anti-Digeorge Syndrome Critical Region 8 antibody
Anti-DGCR8 antibody
Anti-C22orf12 antibody
Anti-DGCRK6 antibody
Anti-LP4941 antibody

Information sourced from Uniprot.org

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