• Western blot of jurkat cell lysate showing specific immunolabeling of the ~34 kDa REDD1 protein phosphorylated at Thr23/25 in the first lane (-). Phosphospecificity is shown in the second lane (+) where Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-DDIT4-Thr23/25 antibody (STJA0003765)

SKU:
STJA0003765-100

£454.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Non-Human Primates
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-REDD1-Thr23/25 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDIT4
Gene ID: 54541
Uniprot ID: DDIT4_HUMAN
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr23/25 conjugated to KLH.
Tissue Specificity Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level).
Post Translational Modifications Phosphorylated by GSK3B.this promotes proteasomal degradation. Polyubiquitinated by a DCX (DDB1-CUL4A-RBX1) E3 ubiquitin-protein ligase complex with BTRC as substrate-recognition component, leading to its proteasomal degradation.
Function Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type.it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes. Required for mTORC1-mediated defense against viral protein synthesis and virus replication. Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death.
Protein Name Dna Damage-Inducible Transcript 4 Protein
Hif-1 Responsive Protein Rtp801
Protein Regulated In Development And Dna Damage Response 1
Redd-1
Database Links Reactome: R-HSA-5628897
Cellular Localisation Mitochondrion
Cytoplasm
Cytosol
Alternative Antibody Names Anti-Dna Damage-Inducible Transcript 4 Protein antibody
Anti-Hif-1 Responsive Protein Rtp801 antibody
Anti-Protein Regulated In Development And Dna Damage Response 1 antibody
Anti-Redd-1 antibody
Anti-DDIT4 antibody
Anti-REDD1 antibody
Anti-RTP801 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance