| Host: | Rabbit |
| Applications: | WB/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal anti-Phospho-Disabled homolog 1-Tyr232 (199-248 aa) for use in WB, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IF 1:200-1:1000ELISA 1:20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | DAB1 |
| Gene ID: | 1600 |
| Uniprot ID: | DAB1_HUMAN |
| Immunogen Region: | 199-248 aa |
| Specificity: | Phospho-Dab1 (Y232) Polyclonal Antibody detects endogenous levels of Dab1 protein only when phosphorylated at Y232. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human Dab1 around the phosphorylation site of Tyr232 at the amino acid range 199-248 |
| Post Translational Modifications | Phosphorylated by FYN on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons. Also phosphorylated on Ser-524 independently of reelin signaling. Ubiquitinated by various cullin-5-RING E3 ubiquitin-protein ligase complexes (ECS complexes) following ligand-binding and phosphorylation, leading to its degradation. Ubiquitinated by the ECS(SOCS7) complex in the cortical plate of the developing cerebral cortex following ligand-binding and phosphorylation by FYN, leading to its degradation by the proteasome. Recognized by ZSWIM8 through a disorder targets misorder mechanism that eliminates misfolded DAB1 via ubiquitination and proteasomal degradation. |
| Function | Signaling adapter of the reelin-mediated signaling pathway, which regulates the migration and differentiation of postmitotic neurons during brain development. Mediates intracellular transduction of Reelin signaling following reelin (RELN)-binding to its receptor: acts by docking proteins through its phosphotyrosine residues and PID domain. |
| Protein Name | Disabled Homolog 1 |
| Database Links | Reactome: R-HSA-8866376 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Disabled Homolog 1 antibodyAnti-DAB1 antibody |
Information sourced from Uniprot.org
