• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Catenin-beta (Phospho-Ser37) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from 293 cells, using Catenin-beta (Phospho-Ser37) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Catenin-Beta (S37) Polyclonal Antibody diluted at 1：2000
  • Western blot analysis of 293 cells using Phospho-Catenin-Beta (S37) Polyclonal Antibody diluted at 1：2000
  • Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, Catenin-Beta (phospho Ser37) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-kidney tissue. 1, Catenin-Beta (phospho Ser37) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, Catenin-Beta (phospho Ser37) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, Catenin-Beta (phospho Ser37) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, Catenin-Beta (phospho Ser37) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of human-kidney tissue. 1, Catenin-Beta (phospho Ser37) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-kidney tissue. 1, Catenin-Beta (phospho Ser37) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-liver-cancer tissue. 1, Catenin-Beta (phospho Ser37) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of human-liver-cancer tissue. 1, Catenin-Beta (phospho Ser37) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-CTNNB1-Ser37 antibody (3-52 aa) (STJ90207)

SKU:
STJ90207

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Catenin beta-1-Ser37 (3-52 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CTNNB1
Gene ID: 1499
Uniprot ID: CTNB1_HUMAN
Immunogen Region: 3-52 aa
Specificity: Phospho-Catenin-Beta (S37) Polyclonal Antibody detects endogenous levels of Catenin-Beta protein only when phosphorylated at S37.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Catenin-beta around the phosphorylation site of Ser37 at the amino acid range 3-52
Post Translational Modifications Phosphorylation at Ser-552 by AMPK promotes stabilization of the protein, enhancing TCF/LEF-mediated transcription. Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33. Phosphorylated by NEK2. EGF stimulates tyrosine phosphorylation. Phosphorylated on Ser-33 and Ser-37 by HIPK2 and GSK3B, this phosphorylation triggers proteasomal degradation. Phosphorylation on Ser-191 and Ser-246 by CDK5. Phosphorylation by CDK2 regulates insulin internalization. Phosphorylation by PTK6 at Tyr-64, Tyr-142, Tyr-331 and/or Tyr-333 with the predominant site at Tyr-64 is not essential for inhibition of transcriptional activity. Phosphorylation by SRC at Tyr-333 promotes interaction with isoform M2 of PKM (PKM2).promoting transcription activation. Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation. Ubiquitinated and degraded following interaction with SOX9. S-nitrosylation at Cys-619 within adherens junctions promotes VEGF-induced, NO-dependent endothelial cell permeability by disrupting interaction with E-cadherin, thus mediating disassembly adherens junctions. O-glycosylation at Ser-23 decreases nuclear localization and transcriptional activity, and increases localization to the plasma membrane and interaction with E-cadherin CDH1. Deacetylated at Lys-49 by SIRT1. Phosphorylated at Thr-556 by herpes virus 1/HHV-1 leading to CTNNB1 inhibition.
Function Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML. Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle. Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling. Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, via promoting the transcription of differentiation factors such as LEF1, BMP2 and BMP4. Activity is repressed in a MSX1-mediated manner at the bell stage of mesenchymal tooth germ formation which prevents premature differentiation of odontoblasts.
Protein Name Catenin Beta-1
Beta-Catenin
Database Links Reactome: R-HSA-195253
Reactome: R-HSA-196299
Reactome: R-HSA-201681
Reactome: R-HSA-201722
Reactome: R-HSA-3134973
Reactome: R-HSA-351906
Reactome: R-HSA-3769402
Reactome: R-HSA-381771
Reactome: R-HSA-4086398
Reactome: R-HSA-418990
Reactome: R-HSA-4411364
Reactome: R-HSA-4641262
Reactome: R-HSA-5218920
Reactome: R-HSA-525793
Reactome: R-HSA-5339716
Reactome: R-HSA-5358747
Reactome: R-HSA-5358749
Reactome: R-HSA-5358751
Reactome: R-HSA-5358752
Reactome: R-HSA-5626467
Reactome: R-HSA-8853884
Reactome: R-HSA-8876493
Reactome: R-HSA-8951430
Reactome: R-HSA-9733709
Reactome: R-HSA-9754189
Reactome: R-HSA-9762292
Reactome: R-HSA-9764302
Reactome: R-HSA-9793380
Reactome: R-HSA-9796292
Reactome: R-HSA-9823730
Reactome: R-HSA-9824272
Reactome: R-HSA-9833576
Cellular Localisation Cytoplasm
Nucleus
Cytoskeleton
Cell Junction
Adherens Junction
Cell Membrane
Microtubule Organizing Center
Centrosome
Spindle Pole
Synapse
Cilium Basal Body
Colocalized With Rapgef2 And Tjp1 At Cell-Cell Contacts
Cytoplasmic When It Is Un-Stable (Highly Phosphorylated) Or Bound To Cdh1
Translocates To The Nucleus When It Is Stabilized (Low Level Of Phosphorylation)
Interaction With Glis2 And Muc1 Promotes Nuclear Translocation
Interaction With Emd Inhibits Nuclear Localization
The Majority Of Beta-Catenin Is Localized To The Cell Membrane
In Interphase
Colocalizes With Crocc Between Cep250 Puncta At The Proximal End Of Centrioles
And This Localization Is Dependent On Crocc And Cep250
In Mitosis
When Nek2 Activity Increases
It Localizes To Centrosomes At Spindle Poles Independent Of Crocc
Colocalizes With Cdk5 In The Cell-Cell Contacts And Plasma Membrane Of Undifferentiated And Differentiated Neuroblastoma Cells
Interaction With Fam53b Promotes Translocation To The Nucleus
Alternative Antibody Names Anti-Catenin Beta-1 antibody
Anti-Beta-Catenin antibody
Anti-CTNNB1 antibody
Anti-CTNNB antibody
Anti-OK antibody
Anti-SW-cl.35 antibody
Anti-PRO2286 antibody

Information sourced from Uniprot.org

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