Anti-Phospho-CSK-Ser364 antibody (330-379 aa) (STJ90456)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Csk around the phosphorylation site of Ser364 at the amino acid range 330-379
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Tyrosine-protein kinase CSK-Ser364 (330-379 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CSK |
| Gene ID | 1445 |
| Uniprot ID | CSK_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Csk around the phosphorylation site of Ser364 at the amino acid range 330-379 |
| Immunogen Region | 330-379 aa |
| Specificity | Phospho-Csk (S364) Polyclonal Antibody detects endogenous levels of Csk protein only when phosphorylated at S364. |
Additional Info
| Post Translational Modifications | Phosphorylated at Ser-364 by PKA, leading to increased activity. Autophosphorylated. |
| Function | Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN, CSK or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK. |
| Protein Name | Tyrosine-Protein Kinase CskC-Src KinaseProtein-Tyrosine Kinase Cyl |
| Database Links | Reactome: R-HSA-180292Reactome: R-HSA-202427Reactome: R-HSA-354192Reactome: R-HSA-389948Reactome: R-HSA-5674135Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-9013407Reactome: R-HSA-9649948Reactome: R-HSA-9656223Reactome: R-HSA-9706369 |
| Cellular Localisation | CytoplasmCell MembraneMainly CytoplasmicAlso Present In Lipid Rafts |
| Alternative Antibody Names | Anti-Tyrosine-Protein Kinase Csk antibodyAnti-C-Src Kinase antibodyAnti-Protein-Tyrosine Kinase Cyl antibodyAnti-CSK antibody |
Information sourced from Uniprot.org