• Western blot analysis of lysates from COS7 cells treated with anisomycin 25ug/ml 30', using CRYAB (Phospho-Ser45) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human heart, using CRYAB (Phospho-Ser45) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CRYAB-Ser45 antibody (21-70 aa) (STJ90896)

SKU:
STJ90896

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Alpha-crystallin B chain-crystallin-Ser45 (21-70 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CRYAB
Gene ID: 1410
Uniprot ID: CRYAB_HUMAN
Immunogen Region: 21-70 aa
Specificity: Phospho-Crystallin-Alpha B (S45) Polyclonal Antibody detects endogenous levels of Crystallin-Alpha B protein only when phosphorylated at S45.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CRYAB around the phosphorylation site of Ser45 at the amino acid range 21-70
Function May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome.
Protein Name Alpha-Crystallin B Chain
Alpha(B-Crystallin
Heat Shock Protein Beta-5
Hspb5
Renal Carcinoma Antigen Ny-Ren-27
Rosenthal Fiber Component
Database Links Reactome: R-HSA-3371571
Cellular Localisation Cytoplasm
Nucleus
Secreted
Lysosome
Translocates To The Nucleus During Heat Shock And Resides In Sub-Nuclear Structures Known As Sc35 Speckles Or Nuclear Splicing Speckles
Localizes At The Z-Bands And The Intercalated Disk In Cardiomyocytes
Can Be Secreted
The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion
Alternative Antibody Names Anti-Alpha-Crystallin B Chain antibody
Anti-Alpha(B-Crystallin antibody
Anti-Heat Shock Protein Beta-5 antibody
Anti-Hspb5 antibody
Anti-Renal Carcinoma Antigen Ny-Ren-27 antibody
Anti-Rosenthal Fiber Component antibody
Anti-CRYAB antibody
Anti-CRYA2 antibody
Anti-HSPB5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance