• Western blot analysis of extracts of K562 cells, using Phospho-CRK-Y221 antibody (STJ117920) at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of lysates from K562 cells, using Phospho-CRK/CRKII-Y221 Rabbit polyclonal antibody (STJ117920) at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-CRK-Y221 antibody (STJ117920)

SKU:
STJ117920

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-CRK-Y221 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CRK
Gene ID: 1398
Uniprot ID: CRK_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y221 of human CRK/CRKII (NP_058431.2).
Immunogen Sequence: GPYAQ
Post Translational Modifications Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway. Isoform Crk-II: Phosphorylated by KIT. Proline isomerization at Pro-237 by PPIA acts as a switch between two conformations: an autoinhibitory conformation in the cis form, where the tandem SH3 domains interact intramolecularly, and an activated conformation in the trans form.
Function Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Isoform Crk-II: Regulates cell adhesion, spreading and migration. Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.
Protein Name Adapter Molecule Crk
Proto-Oncogene C-Crk
P38
Database Links Reactome: R-HSA-170984
Reactome: R-HSA-186763
Reactome: R-HSA-2029482
Reactome: R-HSA-372708
Reactome: R-HSA-4420097
Reactome: R-HSA-8849471
Reactome: R-HSA-8875555
Reactome: R-HSA-8875656
Reactome: R-HSA-912631
Reactome: R-HSA-9664422
Cellular Localisation Cytoplasm
Cell Membrane
Translocated To The Plasma Membrane Upon Cell Adhesion
Alternative Antibody Names Anti-Adapter Molecule Crk antibody
Anti-Proto-Oncogene C-Crk antibody
Anti-P38 antibody
Anti-CRK antibody

Information sourced from Uniprot.org

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