Anti-Phospho-CRK-Tyr221 antibody [R04-6L8] (STJA0036028)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human Phospho-CrkII (Tyr221)
General Information
| Short Description | Rabbit monoclonal anti-Phospho-CRK-Tyr221 for use in WB, ICC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IF |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R04-6L8 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IF 1:50-1:200 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | CRK |
| Gene ID | 1398 |
| Uniprot ID | CRK_HUMAN |
| Immunogen | A synthesized peptide derived from human Phospho-CrkII (Tyr221) |
Additional Info
| Post Translational Modifications | Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway. Isoform Crk-II: Phosphorylated by KIT. Proline isomerization at Pro-237 by PPIA acts as a switch between two conformations: an autoinhibitory conformation in the cis form, where the tandem SH3 domains interact intramolecularly, and an activated conformation in the trans form. |
| Function | Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Isoform Crk-II: Regulates cell adhesion, spreading and migration. Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. |
| Protein Name | Adapter Molecule CrkProto-Oncogene C-CrkP38 |
| Database Links | Reactome: R-HSA-170984Reactome: R-HSA-186763Reactome: R-HSA-2029482Reactome: R-HSA-372708Reactome: R-HSA-4420097Reactome: R-HSA-8849471Reactome: R-HSA-8875555Reactome: R-HSA-8875656Reactome: R-HSA-912631Reactome: R-HSA-9664422 |
| Cellular Localisation | CytoplasmCell MembraneTranslocated To The Plasma Membrane Upon Cell Adhesion |
| Alternative Antibody Names | Anti-Adapter Molecule Crk antibodyAnti-Proto-Oncogene C-Crk antibodyAnti-P38 antibodyAnti-CRK antibody |
Information sourced from Uniprot.org