• Immunofluorescence analysis of HUVEC cells, using CrkII (Phospho-Tyr221) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from COS7 cells, using CrkII (Phospho-Tyr221) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Crk II (Y221) Polyclonal Antibody

Anti-Phospho-CRK-Tyr221 antibody (187-236 aa) (STJ90687)

SKU:
STJ90687

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Adapter molecule crk-Tyr221 (187-236 aa) is suitable for use in Western Blot, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CRK
Gene ID: 1398
Uniprot ID: CRK_HUMAN
Immunogen Region: 187-236 aa
Specificity: Phospho-Crk II (Y221) Polyclonal Antibody detects endogenous levels of Crk II protein only when phosphorylated at Y221.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CrkII around the phosphorylation site of Tyr221 at the amino acid range 187-236
Function Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Isoform Crk-II: Regulates cell adhesion, spreading and migration. Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.
Protein Name Adapter Molecule Crk
Proto-Oncogene C-Crk
P38
Database Links Reactome: R-HSA-170984
Reactome: R-HSA-186763
Reactome: R-HSA-2029482
Reactome: R-HSA-372708
Reactome: R-HSA-4420097
Reactome: R-HSA-8849471
Reactome: R-HSA-8875555
Reactome: R-HSA-8875656
Reactome: R-HSA-912631
Reactome: R-HSA-9664422
Cellular Localisation Cytoplasm
Cell Membrane
Translocated To The Plasma Membrane Upon Cell Adhesion
Alternative Antibody Names Anti-Adapter Molecule Crk antibody
Anti-Proto-Oncogene C-Crk antibody
Anti-P38 antibody
Anti-CRK antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance