• Western blot analysis of lysates from NIH/3T3 cells treated with starved 24h, using ICK (Phospho-Tyr159) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of 3T3 cells using Phospho-Intestinal Cell Kinase (Y159) Polyclonal Antibody

Anti-Phospho-CILK1-Tyr159 antibody (125-174 aa) (STJ90581)

SKU:
STJ90581

Current Stock:
Host: Rabbit
Applications: WB/ELISA/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Serine/threonine-protein kinase ICK-Tyr159 (125-174 aa) is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC-P 1:50-300
ELISA 2000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CILK1
Gene ID: 22858
Uniprot ID: CILK1_HUMAN
Immunogen Region: 125-174 aa
Specificity: Phospho-Intestinal Cell Kinase (Y159) Polyclonal Antibody detects endogenous levels of Intestinal Cell Kinase protein only when phosphorylated at Y159.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ICK around the phosphorylation site of Tyr159 at the amino acid range 125-174
Post Translational Modifications Autophosphorylated on serine and threonine residues. Phosphorylation at Thr-157 increases kinase activity.
Function Required for ciliogenesis. Phosphorylates KIF3A. Involved in the control of ciliary length. Regulates the ciliary localization of SHH pathway components as well as the localization of IFT components at ciliary tips. May play a key role in the development of multiple organ systems and particularly in cardiac development. Regulates intraflagellar transport (IFT) speed and negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner and this regulation requires its kinase activity.
Protein Name Serine/Threonine-Protein Kinase Ick
Ciliogenesis Associated Kinase 1
Intestinal Cell Kinase
Hick
Laryngeal Cancer Kinase 2
Lck2
Mak-Related Kinase
Mrk
Cellular Localisation Nucleus
Cytoplasm
Cytosol
Cell Projection
Cilium
Cytoskeleton
Cilium Basal Body
Also Found At The Ciliary Tip
Nuclear Localization Has Been Observed With A Gfp-Tagged Construct In Transfected Hela Cells
Isoform 2: Cytoplasm
Predominant Cytoplasmic Localization Has Been Observed With A N-Terminally Gfp-Tagged Construct
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Ick antibody
Anti-Ciliogenesis Associated Kinase 1 antibody
Anti-Intestinal Cell Kinase antibody
Anti-Hick antibody
Anti-Laryngeal Cancer Kinase 2 antibody
Anti-Lck2 antibody
Anti-Mak-Related Kinase antibody
Anti-Mrk antibody
Anti-CILK1 antibody
Anti-ICK antibody
Anti-KIAA0936 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance