• Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-CFTR-Ser737 antibody (STJ193221)

SKU:
STJ193221

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Cystic fibrosis transmembrane conductance regulator-Ser737 is suitable for use in Immunohistochemistry, Immunofluorescence and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC-P 1:50-200
WB 1:500-2000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CFTR
Gene ID: 1080
Uniprot ID: CFTR_HUMAN
Specificity: This antibody detects endogenous phospho levels of CFTR (Phospho-Ser737) at Human:S737, Mouse:S732, Rat:S732
Immunogen: Synthesized peptide derived from the human CFTR (Phospho-Ser737)
Post Translational Modifications N-glycosylated. Phosphorylated.cAMP treatment promotes phosphorylation and activates the channel. Dephosphorylation decreases the ATPase activity (in vitro). Phosphorylation at PKA sites activates the channel. Phosphorylation at PKC sites enhances the response to phosphorylation by PKA. Phosphorylated by AMPK.this inhibits channel activity. Ubiquitinated, leading to its degradation in the lysosome. Deubiquitination by USP10 in early endosomes enhances its endocytic recycling to the cell membrane. Ubiquitinated by RNF185 during ER stress. Ubiquitinated by MARCHF2.
Function Epithelial ion channel that plays an important role in the regulation of epithelial ion and water transport and fluid homeostasis. Mediates the transport of chloride ions across the cell membrane. Channel activity is coupled to ATP hydrolysis. The ion channel is also permeable to HCO(3)(-).selectivity depends on the extracellular chloride concentration. Exerts its function also by modulating the activity of other ion channels and transporters. Plays an important role in airway fluid homeostasis. Contributes to the regulation of the pH and the ion content of the airway surface fluid layer and thereby plays an important role in defense against pathogens. Modulates the activity of the epithelial sodium channel (ENaC) complex, in part by regulating the cell surface expression of the ENaC complex. Inhibits the activity of the ENaC channel containing subunits SCNN1A, SCNN1B and SCNN1G. Inhibits the activity of the ENaC channel containing subunits SCNN1D, SCNN1B and SCNN1G, but not of the ENaC channel containing subunits SCNN1A, SCNN1B and SCNN1G. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the transporter SLC4A7. Can inhibit the chloride channel activity of ANO1. Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation.
Protein Name Cystic Fibrosis Transmembrane Conductance Regulator
Cftr
Atp-Binding Cassette Sub-Family C Member 7
Channel Conductance-Controlling Atpase
Camp-Dependent Chloride Channel
Database Links Reactome: R-HSA-382556
Reactome: R-HSA-5627083
Reactome: R-HSA-5678895
Reactome: R-HSA-5689880
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-9013406
Reactome: R-HSA-9613829
Reactome: R-HSA-9615710
Reactome: R-HSA-9646399
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Early Endosome Membrane
Cell Membrane
Recycling Endosome Membrane
Endoplasmic Reticulum Membrane
Nucleus
The Channel Is Internalized From The Cell Surface Into An Endosomal Recycling Compartment
From Where It Is Recycled To The Cell Membrane
In The Oviduct And Bronchus
Detected On The Apical Side Of Epithelial Cells
But Not Associated With Cilia
In Sertoli Cells
A Processed Product Is Detected In The Nucleus
Er Stress Induces Gorasp2-Mediated Unconventional (Er/Golgi-Independent) Trafficking Of Core-Glycosylated Cftr To Cell Membrane
Alternative Antibody Names Anti-Cystic Fibrosis Transmembrane Conductance Regulator antibody
Anti-Cftr antibody
Anti-Atp-Binding Cassette Sub-Family C Member 7 antibody
Anti-Channel Conductance-Controlling Atpase antibody
Anti-Camp-Dependent Chloride Channel antibody
Anti-CFTR antibody
Anti-ABCC7 antibody

Information sourced from Uniprot.org

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