Host: | Rabbit |
Applications: | WB/IHC/IF/ELISA |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-Phospho-Cyclin-dependent kinase inhibitor 1-Thr145 (111-160 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration: | 1 mg/mL |
Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | CDKN1A |
Gene ID: | 1026 |
Uniprot ID: | CDN1A_HUMAN |
Immunogen Region: | 111-160 aa |
Specificity: | Phospho-p21 (T145) Polyclonal Antibody detects endogenous levels of p21 protein only when phosphorylated at T145. |
Immunogen: | The antiserum was produced against synthesized peptide derived from the human p21 Cip1 around the phosphorylation site of Thr145 at the amino acid range 111-160 |
Post Translational Modifications | Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex. Phosphorylation of Thr-145 by PIM2 enhances CDKN1A stability and inhibits cell proliferation. Phosphorylation of Thr-145 by PIM1 results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. UV radiation-induced phosphorylation at Thr-80 by LKB1 and at Ser-146 by NUAK1 leads to its degradation. Ubiquitinated by MKRN1.leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. Ubiquitination at Ser-2 leads to degradation by the proteasome pathway. Ubiquitinated by RNF114.leading to proteasomal degradation. Acetylation leads to protein stability. Acetylated in vitro on Lys-141, Lys-154, Lys-161 and Lys-163. Deacetylation by HDAC1 is prevented by competitive binding of C10orf90/FATS to HDAC1. |
Function | Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest. Involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Also involved in p53-independent DNA damage-induced G2 arrest mediated by CREB3L1 in astrocytes and osteoblasts. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding. |
Protein Name | Cyclin-Dependent Kinase Inhibitor 1 Cdk-Interacting Protein 1 Melanoma Differentiation-Associated Protein 6 Mda-6 P21 |
Database Links | Reactome: R-HSA-187577 Reactome: R-HSA-198323 Reactome: R-HSA-2559582 Reactome: R-HSA-2559586 Reactome: R-HSA-5674400 Reactome: R-HSA-6785807 Reactome: R-HSA-6804116 Reactome: R-HSA-69202 Reactome: R-HSA-69231 Reactome: R-HSA-69563 Reactome: R-HSA-69656 Reactome: R-HSA-69895 Reactome: R-HSA-8852276 Reactome: R-HSA-8866911 Reactome: R-HSA-8878166 Reactome: R-HSA-8941855 Reactome: R-HSA-8951664 Reactome: R-HSA-9616222 Reactome: R-HSA-9617828 Reactome: R-HSA-9661069 Reactome: R-HSA-9702518 Reactome: R-HSA-9703465 Reactome: R-HSA-9755511 |
Cellular Localisation | Cytoplasm Nucleus |
Alternative Antibody Names | Anti-Cyclin-Dependent Kinase Inhibitor 1 antibody Anti-Cdk-Interacting Protein 1 antibody Anti-Melanoma Differentiation-Associated Protein 6 antibody Anti-Mda-6 antibody Anti-P21 antibody Anti-CDKN1A antibody Anti-CAP20 antibody Anti-CDKN1 antibody Anti-CIP1 antibody Anti-MDA6 antibody Anti-PIC1 antibody Anti-SDI1 antibody Anti-WAF1 antibody |
Information sourced from Uniprot.org