• Western blot analysis of extracts of various cell lines, using Phospho-Cdk1/2-T14 rabbit monoclonal antibody (STJ11102590) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by Hydroxyurea (4 Mm) at 37 °C for 20 hours or treated by Nocodazole (100 ng/mL) at 37 °C for 24 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using Phospho-Cdk1/2-T14 Rabbit monoclonal antibody (STJ11102590) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by Hydroxyurea (4 Mm) at 37 °C for 20 hours or treated by Nocodazole (100 ng/mL) at 37 °C for 24 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-CDK1/CDK2-T14 antibody [S0MR] (STJ11102590)

SKU:
STJ11102590

£146.50 - £642.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDK1
CDK2
Gene ID: 983
1017
Uniprot ID: CDK1_HUMAN
CDK2_HUMAN
Specificity: A synthetic phosphorylated peptide around T14 of human Cdk1/2CDK1 (P06493).
Immunogen Sequence: EGTYG
Tissue Specificity
Alternative Antibody Names Anti-Cyclin-Dependent Kinase 1 antibody
Anti-Cdk1 antibody
Anti-Cell Division Control Protein 2 Homolog antibody
Anti-Cell Division Protein Kinase 1 antibody
Anti-P34 Protein Kinase antibody
Anti-CDK1 antibody
Anti-CDC2 antibody
Anti-CDC28A antibody
Anti-CDKN1 antibody
Anti-P34CDC2 antibody
Anti-Cyclin-Dependent Kinase 2 antibody
Anti-Cell Division Protein Kinase 2 antibody
Anti-P33 Protein Kinase antibody
Anti-CDK2 antibody
Anti-CDKN2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance