• Western blot analysis of extracts of HT-29 cells, using Phospho-cdc25C-S216 antibody (STJ29343). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
  • Immunohistochemistry analysis of Phospho-cdc25C-S216 in paraffin-embedded rat brain tissue using Phospho-cdc25C-S216 Rabbit polyclonal antibody (STJ29343) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Phospho-cdc25C-S216 in paraffin-embedded rat colon tissue using Phospho-cdc25C-S216 Rabbit polyclonal antibody (STJ29343) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Phospho-cdc25C-S216 in paraffin-embedded mouse liver tissue using Phospho-cdc25C-S216 Rabbit polyclonal antibody (STJ29343) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Phospho-cdc25C-S216 in paraffin-embedded human cervix cancer tissue using Phospho-cdc25C-S216 Rabbit polyclonal antibody (STJ29343) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Phospho-cdc25C-S216 in paraffin-embedded mouse intestin tissue using Phospho-cdc25C-S216 Rabbit polyclonal antibody (STJ29343) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Phospho-cdc25C-S216 in paraffin-embedded human colon tissue using Phospho-cdc25C-S216 Rabbit polyclonal antibody (STJ29343) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from HT-29 cells, using Phospho-cdc25C-S216 Rabbit polyclonal antibody (STJ29343) at 1:400 dilution. HT-29 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-CDC25C-S216 antibody (STJ29343)

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STJ29343

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-cdc25C-S216 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDC25C
Gene ID: 995
Uniprot ID: MPIP3_HUMAN
Immunogen: A synthetic phosphorylated peptide around S216 of human cdc25C (NP_001781.2).
Immunogen Sequence: SPSMP
Post Translational Modifications Phosphorylated by CHEK1 and MAPK14 at Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-191 promotes nuclear translocation. Ser-198 is a minor phosphorylation site. Was initially reported to be phosphorylated by PLK3 at Ser-216. However, such phosphorylation by PLK3 was not confirmed by other groups. Phosphorylation at Thr-48, Thr-67, Ser-122, Thr-130, Ser-168 and Ser-214 occurs at G2 and G2-M transition and is probably catalyzed by CDK1. Ser-168 phosphorylation levels are lower than those at the other 5 CDK1 sites. Phosphorylation by CDK1 leads to increased activity.
Function Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Protein Name M-Phase Inducer Phosphatase 3
Dual Specificity Phosphatase Cdc25c
Database Links Reactome: R-HSA-156711
Reactome: R-HSA-176187
Reactome: R-HSA-5625740
Reactome: R-HSA-6804114
Reactome: R-HSA-6804115
Reactome: R-HSA-69273
Reactome: R-HSA-75035
Reactome: R-HSA-8862803
Cellular Localisation Nucleus
Alternative Antibody Names Anti-M-Phase Inducer Phosphatase 3 antibody
Anti-Dual Specificity Phosphatase Cdc25c antibody
Anti-CDC25C antibody

Information sourced from Uniprot.org

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