• Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30', using CDC25B (Phospho-Ser323) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of VEC cells using Phospho-Cdc25B (S323) Polyclonal Antibody diluted at 1：1000
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of various cells using Phospho-Cdc25B (S323) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-CDC25B-Ser323 antibody (289-338 aa) (STJ90217)

SKU:
STJ90217

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-M-phase inducer phosphatase 2-Ser323 (289-338 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDC25B
Gene ID: 994
Uniprot ID: MPIP2_HUMAN
Immunogen Region: 289-338 aa
Specificity: Phospho-Cdc25B (S323) Polyclonal Antibody detects endogenous levels of Cdc25B protein only when phosphorylated at S323.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CDC25B around the phosphorylation site of Ser323 at the amino acid range 289-338
Function Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Protein Name M-Phase Inducer Phosphatase 2
Dual Specificity Phosphatase Cdc25b
Database Links Reactome: R-HSA-69273
Reactome: R-HSA-69656
Reactome: R-HSA-8862803
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle Pole
Alternative Antibody Names Anti-M-Phase Inducer Phosphatase 2 antibody
Anti-Dual Specificity Phosphatase Cdc25b antibody
Anti-CDC25B antibody
Anti-CDC25HU2 antibody

Information sourced from Uniprot.org

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