Anti-Phospho-CD226-Ser329 antibody (287-336 aa) (STJ91074)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human CD226/DNAM-1 around the phosphorylation site of Ser329 at the amino acid range 287-336
General Information
| Short Description | Rabbit polyclonal anti-Phospho-CD226 antigen-Ser329 (287-336 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Monkey |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:40000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CD226 |
| Gene ID | 10666 |
| Uniprot ID | CD226_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human CD226/DNAM-1 around the phosphorylation site of Ser329 at the amino acid range 287-336 |
| Immunogen Region | 287-336 aa |
| Specificity | Phospho-DNAM-1 (S329) Polyclonal Antibody detects endogenous levels of DNAM-1 protein only when phosphorylated at S329. |
Additional Info
| Post Translational Modifications | PKC-mediated phosphorylation at Ser-329 is required for lipid raft recruitment. Phosphorylation of Tyr-322 requires association with lipid rafts and allows GRB2 binding and activation of subsequent signaling. |
| Function | Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells. Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells. Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes. |
| Protein Name | Cd226 AntigenDnax Accessory Molecule 1Dnam-1Cd Antigen Cd226 |
| Database Links | Reactome: R-HSA-198933 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinLocalizes To Lipid Rafts |
| Alternative Antibody Names | Anti-Cd226 Antigen antibodyAnti-Dnax Accessory Molecule 1 antibodyAnti-Dnam-1 antibodyAnti-Cd Antigen Cd226 antibodyAnti-CD226 antibodyAnti-DNAM1 antibody |
Information sourced from Uniprot.org