Anti-Phospho-CCND3-Thr283 antibody (243-292 aa) (STJ90240)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Cyclin D3 around the phosphorylation site of Thr283 at the amino acid range 243-292
General Information
| Short Description | Rabbit polyclonal anti-Phospho-G1/S-specific cyclin-D3-Thr283 (243-292 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CCND3 |
| Gene ID | 896 |
| Uniprot ID | CCND3_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Cyclin D3 around the phosphorylation site of Thr283 at the amino acid range 243-292 |
| Immunogen Region | 243-292 aa |
| Specificity | Phospho-Cyclin D3 (T283) Polyclonal Antibody detects endogenous levels of Cyclin D3 protein only when phosphorylated at T283. |
Additional Info
| Function | Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Shows transcriptional coactivator activity with ATF5 independently of CDK4. |
| Protein Name | G1/S-Specific Cyclin-D3 |
| Database Links | Reactome: R-HSA-381340Reactome: R-HSA-5687128Reactome: R-HSA-69231Reactome: R-HSA-8934593Reactome: R-HSA-9661069Reactome: R-HSA-9754119 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-G1/S-Specific Cyclin-D3 antibodyAnti-CCND3 antibody |
Information sourced from Uniprot.org