• Western blot analysis of lysates from 293 cells treated with Etoposide 25uM 60', using Caspase 6 (Phospho-Ser257) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Caspase 6 (Phospho-Ser257) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunofluorescence analysis of HUVEC cells, using Caspase 6 (Phospho-Ser257) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of 293T cells using Phospho-Caspase-6 (S257) Polyclonal Antibody diluted at 1:1000

Anti-Phospho-CASP6-Ser257 antibody (223-272 aa) (STJ90204)

SKU:
STJ90204

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Caspase-6-Ser257 (223-272 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CASP6
Gene ID: 839
Uniprot ID: CASP6_HUMAN
Immunogen Region: 223-272 aa
Specificity: Phospho-Caspase-6 (S257) Polyclonal Antibody detects endogenous levels of Caspase-6 protein only when phosphorylated at S257.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Caspase 6 around the phosphorylation site of Ser257 at the amino acid range 223-272
Function Cysteine protease that plays essential roles in programmed cell death, axonal degeneration, development and innate immunity. Acts as a non-canonical executioner caspase during apoptosis: localizes in the nucleus and cleaves the nuclear structural protein NUMA1 and lamin A/LMNA thereby inducing nuclear shrinkage and fragmentation. Lamin-A/LMNA cleavage is required for chromatin condensation and nuclear disassembly during apoptotic execution. Acts as a regulator of liver damage by promoting hepatocyte apoptosis: in absence of phosphorylation by AMP-activated protein kinase (AMPK), catalyzes cleavage of BID, leading to cytochrome c release, thereby participating in nonalcoholic steatohepatitis. Cleaves PARK7/DJ-1 in cells undergoing apoptosis. Involved in intrinsic apoptosis by mediating cleavage of RIPK1. Furthermore, cleaves many transcription factors such as NF-kappa-B and cAMP response element-binding protein/CREBBP. Cleaves phospholipid scramblase proteins XKR4 and XKR9. In addition to apoptosis, involved in different forms of programmed cell death. Plays an essential role in defense against viruses by acting as a central mediator of the ZBP1-mediated pyroptosis, apoptosis, and necroptosis (PANoptosis), independently of its cysteine protease activity. PANoptosis is a unique inflammatory programmed cell death, which provides a molecular scaffold that allows the interactions and activation of machinery required for inflammasome/pyroptosis, apoptosis and necroptosis. Mechanistically, interacts with RIPK3 and enhances the interaction between RIPK3 and ZBP1, leading to ZBP1-mediated inflammasome activation and cell death. Plays an essential role in axon degeneration during axon pruning which is the remodeling of axons during neurogenesis but not apoptosis. Regulates B-cell programs both during early development and after antigen stimulation. (Microbial infection) Proteolytically cleaves the N protein of coronoviruses such as MERS-CoV and SARS-CoV. The cleavage of MERS-CoV N-protein leads to two fragments and modulates coronavirus replication by regulating IFN signaling. The two fragments produced by the cleavage interact with IRF3 inhibiting its nuclear translocation after activation and reduce the expression of IFNB and IFN-stimulated genes. The same mechanism seems to be used by other coronaviruses such as SARS-CoV and SARS-CoV-2 to enhance their replication.
Protein Name Caspase-6
Casp-6
Csp-6
Apoptotic Protease Mch-2 Cleaved Into - Caspase-6 Subunit P18
Caspase-6 Subunit P20 - Caspase-6 Subunit P11
Caspase-6 Subunit P10
Database Links Reactome: R-HSA-111465
Reactome: R-HSA-264870
Reactome: R-HSA-352238
Reactome: R-HSA-6803207
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Caspase-6 antibody
Anti-Casp-6 antibody
Anti-Csp-6 antibody
Anti-Apoptotic Protease Mch-2 Cleaved Into - Caspase-6 Subunit P18 antibody
Anti-Caspase-6 Subunit P20 - Caspase-6 Subunit P11 antibody
Anti-Caspase-6 Subunit P10 antibody
Anti-CASP6 antibody
Anti-MCH2 antibody

Information sourced from Uniprot.org

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