| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/IP/ELISA |
| Reactivity: |
Human/Monkey |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Apoptosis regulator Bcl-2-Ser70 (41-90 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300IP 2-5 ug mg/lysateELISA 1:10000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
BCL2 |
| Gene ID: |
596 |
| Uniprot ID: |
BCL2_HUMAN |
| Immunogen Region: |
41-90 aa |
| Specificity: |
Phospho-Bcl-2 (S70) Polyclonal Antibody detects endogenous levels of Bcl-2 protein only when phosphorylated at S70. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human BCL-2 around the phosphorylation site of Ser70 at the amino acid range 41-90 |
| Post Translational Modifications | Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophag. Dephosphorylated by protein phosphatase 2A (PP2A). Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. Monoubiquitinated by PRKN, leading to an increase in its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome. Ubiquitinated by XIAP, leading to its degradation by the proteasome. |
| Function | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function. May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. |
| Protein Name | Apoptosis Regulator Bcl-2 |
| Database Links | Reactome: R-HSA-111447Reactome: R-HSA-111453Reactome: R-HSA-6785807Reactome: R-HSA-844455Reactome: R-HSA-9018519Reactome: R-HSA-9634638 |
| Cellular Localisation | Mitochondrion Outer MembraneSingle-Pass Membrane ProteinNucleus MembraneEndoplasmic Reticulum MembraneCytoplasm |
| Alternative Antibody Names | Anti-Apoptosis Regulator Bcl-2 antibodyAnti-BCL2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
