• Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30' and A549 cells treated with PMA 125ng/ml 30', using p130 Cas (Phospho-Tyr249) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p130 Cas (Phospho-Tyr249) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BCAR1-Tyr249 antibody (215-264 aa) (STJ90858)

SKU:
STJ90858

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Breast cancer anti-estrogen resistance protein 1-Tyr249 (215-264 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BCAR1
Gene ID: 9564
Uniprot ID: BCAR1_HUMAN
Immunogen Region: 215-264 aa
Specificity: Phospho-p130 Cas (Y249) Polyclonal Antibody detects endogenous levels of p130 Cas protein only when phosphorylated at Y249.
Immunogen: The antiserum was produced against synthesized peptide derived from the human p130 Cas around the phosphorylation site of Tyr249 at the amino acid range 215-264
Post Translational Modifications PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif.phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix. Dephosphorylated by PTPN14 at Tyr-128. Phosphorylated by SRC kinase in a EDN1- and PTK2B-mediated manner.phosphorylation strengthens its interaction with BCAR3 as part of the PTK2B/BCAR1/BCAR3/RAP1 signaling pathway.
Function Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration and cell branching. Involved in the BCAR3-mediated inhibition of TGFB signaling.
Protein Name Breast Cancer Anti-Estrogen Resistance Protein 1
Crk-Associated Substrate
Cas Scaffolding Protein Family Member 1
P130cas
Database Links Reactome: R-HSA-186763
Reactome: R-HSA-372708
Reactome: R-HSA-4420097
Reactome: R-HSA-8849471
Cellular Localisation Cell Junction
Focal Adhesion
Cytoplasm
Cell Projection
Axon
Unphosphorylated Form Localizes In The Cytoplasm
Localizes To Focal Adhesion Sites Following Integrin Engagement
Alternative Antibody Names Anti-Breast Cancer Anti-Estrogen Resistance Protein 1 antibody
Anti-Crk-Associated Substrate antibody
Anti-Cas Scaffolding Protein Family Member 1 antibody
Anti-P130cas antibody
Anti-BCAR1 antibody
Anti-CAS antibody
Anti-CASS1 antibody
Anti-CRKAS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance