Anti-Phospho-BAD-Ser134 antibody (100-149 aa) (STJ91070)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human BAD around the phosphorylation site of Ser134 at the amino acid range 100-149
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Bcl2-associated agonist of cell death-Ser134 (100-149 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BAD |
| Gene ID | 572 |
| Uniprot ID | BAD_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human BAD around the phosphorylation site of Ser134 at the amino acid range 100-149 |
| Immunogen Region | 100-149 aa |
| Specificity | Phospho-Bad (S134) Polyclonal Antibody detects endogenous levels of Bad protein only when phosphorylated at S134. |
Additional Info
| Post Translational Modifications | Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Phosphorylation at Ser-99 by PKB/AKT1 is almost completely blocked by the apoptotic C-terminus cleavage product of PKN2 generated by caspases-3 activity during apoptosis. Methylation at Arg-94 and Arg-96 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-99. |
| Function | Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. |
| Protein Name | Bcl2-Associated Agonist Of Cell DeathBadBcl-2-Binding Component 6Bcl-2-Like Protein 8Bcl2-L-8Bcl-Xl/Bcl-2-Associated Death PromoterBcl2 Antagonist Of Cell Death |
| Database Links | Reactome: R-HSA-111447Reactome: R-HSA-111453Reactome: R-HSA-193648Reactome: R-HSA-198323Reactome: R-HSA-5674400 |
| Cellular Localisation | Mitochondrion Outer MembraneCytoplasmColocalizes With Hif3a In The CytoplasmUpon PhosphorylationLocates To The Cytoplasm |
| Alternative Antibody Names | Anti-Bcl2-Associated Agonist Of Cell Death antibodyAnti-Bad antibodyAnti-Bcl-2-Binding Component 6 antibodyAnti-Bcl-2-Like Protein 8 antibodyAnti-Bcl2-L-8 antibodyAnti-Bcl-Xl/Bcl-2-Associated Death Promoter antibodyAnti-Bcl2 Antagonist Of Cell Death antibodyAnti-BAD antibodyAnti-BBC6 antibodyAnti-BCL2L8 antibody |
Information sourced from Uniprot.org