• Western blot analysis of lysates from HepG2 cells treated with Adriamycin 0.5uM 5h, using Ataxin 1 (Phospho-Ser776) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of NIH/3T3 cells, using Ataxin 1 (Phospho-Ser776) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ATXN1-Ser776 antibody (742-791 aa) (STJ90696)

SKU:
STJ90696

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Ataxin-1-Ser776 (742-791 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ATXN1
Gene ID: 6310
Uniprot ID: ATX1_HUMAN
Immunogen Region: 742-791 aa
Specificity: Phospho-Ataxin-1 (S776) Polyclonal Antibody detects endogenous levels of Ataxin-1 protein only when phosphorylated at S776.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Ataxin 1 around the phosphorylation site of Ser776 at the amino acid range 742-791
Post Translational Modifications Ubiquitinated by UBE3A, leading to its degradation by the proteasome. The presence of expanded poly-Gln repeats in spinocerebellar ataxia 1 (SCA1) patients impairs ubiquitination and degradation, leading to accumulation of ATXN1 in neurons and subsequent toxicity. Phosphorylation at Ser-775 increases the pathogenicity of proteins with an expanded polyglutamine tract. Sumoylation is dependent on nuclear localization and phosphorylation at Ser-775. It is reduced in the presence of an expanded polyglutamine tract.
Function Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression. Binds RNA in vitro. May be involved in RNA metabolism. In concert with CIC and ATXN1L, involved in brain development.
Protein Name Ataxin-1
Spinocerebellar Ataxia Type 1 Protein
Cellular Localisation Cytoplasm
Nucleus
Colocalizes With Usp7 In The Nucleus
Alternative Antibody Names Anti-Ataxin-1 antibody
Anti-Spinocerebellar Ataxia Type 1 Protein antibody
Anti-ATXN1 antibody
Anti-ATX1 antibody
Anti-SCA1 antibody

Information sourced from Uniprot.org

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