• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using A-RAF (Phospho-Tyr302) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of lysates from HeLa cells treated with PMA 125ng/ml 30', using A-RAF (Phospho-Tyr302) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunofluorescence analysis of HepG2 cells, using A-RAF (Phospho-Tyr302) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ARAF-Tyr302 antibody (276-325 aa) (STJ91064)

SKU:
STJ91064

£48.00 - £252.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Serine/threonine-protein kinase A-Raf-Tyr302 (276-325 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARAF
Gene ID: 369
Uniprot ID: ARAF_HUMAN
Immunogen Region: 276-325 aa
Specificity: Phospho-A-Raf (Y302) Polyclonal Antibody detects endogenous levels of A-Raf protein only when phosphorylated at Y302.
Immunogen: The antiserum was produced against synthesized peptide derived from the human A-RAF around the phosphorylation site of Tyr302 at the amino acid range 276-325
Post Translational Modifications Dephosphorylation of Ser-214 by the SHOC2-MRAS-PP1c (SMP) complex consisting of SHOC2, GTP-bound M-Ras/MRAS and the catalytic subunit of protein phosphatase 1 (PPP1CA, PPP1CB or PPP1CC).this relieves inactivation and stimulates kinase activity.
Function Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. Phosphorylates PFKFB2. Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.
Protein Name Serine/Threonine-Protein Kinase A-Raf
Proto-Oncogene A-Raf
Proto-Oncogene A-Raf-1
Proto-Oncogene Pks
Database Links Reactome: R-HSA-5673000
Reactome: R-HSA-5674135
Reactome: R-HSA-5675221
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9726840
Reactome: R-HSA-9726842
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase A-Raf antibody
Anti-Proto-Oncogene A-Raf antibody
Anti-Proto-Oncogene A-Raf-1 antibody
Anti-Proto-Oncogene Pks antibody
Anti-ARAF antibody
Anti-ARAF1 antibody
Anti-PKS antibody
Anti-PKS2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance