• Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-ARAF-Ser299 antibody (266-315 aa) (STJ91197)

SKU:
STJ91197

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Serine/threonine-protein kinase A-Raf-Ser299 (266-315 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARAF
Gene ID: 369
Uniprot ID: ARAF_HUMAN
Immunogen Region: 266-315 aa
Specificity: Phospho-A-Raf (S299) Polyclonal Antibody detects endogenous levels of A-Raf protein only when phosphorylated at S299.
Immunogen: The antiserum was produced against synthesized peptide derived from the human A-RAF around the phosphorylation site of Ser299 at the amino acid range 266-315
Function Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. Phosphorylates PFKFB2. Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.
Protein Name Serine/Threonine-Protein Kinase A-Raf
Proto-Oncogene A-Raf
Proto-Oncogene A-Raf-1
Proto-Oncogene Pks
Database Links Reactome: R-HSA-5673000
Reactome: R-HSA-5674135
Reactome: R-HSA-5675221
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9726840
Reactome: R-HSA-9726842
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase A-Raf antibody
Anti-Proto-Oncogene A-Raf antibody
Anti-Proto-Oncogene A-Raf-1 antibody
Anti-Proto-Oncogene Pks antibody
Anti-ARAF antibody
Anti-ARAF1 antibody
Anti-PKS antibody
Anti-PKS2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance