Anti-Phospho-APLF-Ser116 antibody (82-131 aa) (STJ91238)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human APLF around the phosphorylation site of Ser116 at the amino acid range 82-131
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Aprataxin and PNK-like factor-Ser116 (82-131 aa) for use in IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | APLF |
| Gene ID | 200558 |
| Uniprot ID | APLF_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human APLF around the phosphorylation site of Ser116 at the amino acid range 82-131 |
| Immunogen Region | 82-131 aa |
| Specificity | Phospho-APLF (S116) Polyclonal Antibody detects endogenous levels of APLF protein only when phosphorylated at S116. |
Additional Info
| Function | Histone chaperone involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with branched poly-ADP-ribose chains, a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Following recruitment to DNA damage sites, acts as a histone chaperone that mediates histone eviction during DNA repair and promotes recruitment of histone variant MACROH2A1. Also has a nuclease activity: displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. Together with PARP3, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ). Also acts as a negative regulator of cell pluripotency by promoting histone exchange. Required for the embryo implantation during the epithelial to mesenchymal transition in females. |
| Protein Name | Aprataxin And Pnk-Like FactorApurinic-Apyrimidinic Endonuclease AplfPnk And Aptx-Like Fha Domain-Containing ProteinXrcc1-Interacting Protein 1 |
| Database Links | |
| Cellular Localisation | NucleusChromosomeCytoplasmCytosolLocalizes To Dna Damage SitesAccumulates At Single-Strand Breaks And Double-Strand Breaks Via The Pbz-Type Zinc Fingers |
| Alternative Antibody Names | Anti-Aprataxin And Pnk-Like Factor antibodyAnti-Apurinic-Apyrimidinic Endonuclease Aplf antibodyAnti-Pnk And Aptx-Like Fha Domain-Containing Protein antibodyAnti-Xrcc1-Interacting Protein 1 antibodyAnti-APLF antibodyAnti-C2orf13 antibodyAnti-PALF antibodyAnti-XIP1 antibody |
Information sourced from Uniprot.org