| Host: |
Rabbit |
| Applications: |
IHC/IF/WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-ALK tyrosine kinase receptor-Tyr1586 is suitable for use in Immunohistochemistry, Immunofluorescence and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
IHC-P 1:50-200WB 1:500-2000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ALK |
| Gene ID: |
238 |
| Uniprot ID: |
ALK_HUMAN |
| Specificity: |
This antibody detects endogenous levels of ALK at Human:Y1586; Mouse:Y1592, It doesn NA t reacte with total protein. |
| Immunogen: |
Synthesized peptide derived from the human ALK (Phospho-Tyr1586) |
| Post Translational Modifications | Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity. In cells not stimulated by a ligand, receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) dephosphorylates ALK at the sites in ALK that are undergoing autophosphorylation through autoactivation. Phosphorylation at Tyr-1507 is critical for SHC1 association. N-glycosylated. |
| Function | Neuronal receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Also acts as a key thinness protein involved in the resistance to weight gain: in hypothalamic neurons, controls energy expenditure acting as a negative regulator of white adipose tissue lipolysis and sympathetic tone to fine-tune energy homeostasis. Following activation by ALKAL2 ligand at the cell surface, transduces an extracellular signal into an intracellular response. In contrast, ALKAL1 is not a potent physiological ligand for ALK. Ligand-binding to the extracellular domain induces tyrosine kinase activation, leading to activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. ALK activation may also be regulated by pleiotrophin (PTN) and midkine (MDK). PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK. |
| Protein Name | Alk Tyrosine Kinase ReceptorAnaplastic Lymphoma KinaseCd Antigen Cd246 |
| Database Links | Reactome: R-HSA-201556Reactome: R-HSA-9700645Reactome: R-HSA-9717264Reactome: R-HSA-9717301Reactome: R-HSA-9717316Reactome: R-HSA-9717319Reactome: R-HSA-9717323Reactome: R-HSA-9717326Reactome: R-HSA-9717329Reactome: R-HSA-9725370Reactome: R-HSA-9725371 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane Attachment Is Essential For Promotion Of Neuron-Like Differentiation And Cell Proliferation Arrest Through Specific Activation Of The Map Kinase Pathway |
| Alternative Antibody Names | Anti-Alk Tyrosine Kinase Receptor antibodyAnti-Anaplastic Lymphoma Kinase antibodyAnti-Cd Antigen Cd246 antibodyAnti-ALK antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
