| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Phospho-Proline-rich AKT1 substrate 1-Thr246 (207-256 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000ELISA 1:10000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
AKT1S1 |
| Gene ID: |
84335 |
| Uniprot ID: |
AKTS1_HUMAN |
| Immunogen Region: |
207-256 aa |
| Specificity: |
Phospho-PRAS40 (T246) Polyclonal Antibody detects endogenous levels of PRAS40 protein only when phosphorylated at T246. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human Akt1 S1 around the phosphorylation site of Thr246 at the amino acid range 207-256 |
| Post Translational Modifications | Phosphorylated by AKT1.phosphorylation takes place in response to insulin treatment and promotes AKT1S1 interaction with 14-3-3 proteins, leading to relieve its inhibitor activity. Phosphorylated by MTOR following mTORC1 activation, inhibiting AKT1S1 inhibitor activity: phosphorylation by MTOR probably serves as a feedback loop that relieves inhibition from AKT1S1 in response to mTORC1 inactivation. Phosphorylation at Thr-246 by DYRK3 relieves inhibitory function on mTORC1. |
| Function | Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. In absence of insulin and nutrients, AKT1S1 associates with the mTORC1 complex and directly inhibits mTORC1 activity by blocking the MTOR substrate-recruitment site. In response to insulin and nutrients, AKT1S1 dissociates from mTORC1. Its activity is dependent on its phosphorylation state and binding to 14-3-3. May also play a role in nerve growth factor-mediated neuroprotection. |
| Protein Name | Proline-Rich Akt1 Substrate 140 Kda Proline-Rich Akt Substrate |
| Database Links | Reactome: R-HSA-165159Reactome: R-HSA-166208Reactome: R-HSA-198323Reactome: R-HSA-3371571Reactome: R-HSA-5674400 |
| Cellular Localisation | CytoplasmCytosolFound In The Cytosolic Fraction Of The Brain |
| Alternative Antibody Names | Anti-Proline-Rich Akt1 Substrate 1 antibodyAnti-40 Kda Proline-Rich Akt Substrate antibodyAnti-AKT1S1 antibodyAnti-PRAS40 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
