• Western blot analysis of extracts of various cell lines, using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 antibody (STJ11101106) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. C6 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of Phospho-Akt-S473 in paraffin-embedded mouse pancreas using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Rabbit polyclonal antibody (STJ11101106) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat lung using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 rabbit polyclonal antibody (STJ11101106) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Phospho-Akt-S473 in paraffin-embedded human colon carcinoma using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Rabbit polyclonal antibody (STJ11101106) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 rabbit polyclonal antibody (STJ11101106) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Phospho-Akt-S473 in paraffin-embedded rat lung using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Rabbit polyclonal antibody (STJ11101106) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse pancreas using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 rabbit polyclonal antibody (STJ11101106) at dilution of 1:100 (40x lens).
  • Western blot analysis of lysates from Jurkat cells using Phospho-Akt-S473 Rabbit polyclonal antibody (STJ11101106) at 1:500 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.
  • Western blot analysis of lysates from NIH/3T3 cells using Phospho-Akt-S473 Rabbit polyclonal antibody (STJ11101106) at 1:500 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 45s.
  • Western blot analysis of lysates from C6 cells using Phospho-Akt-S473 Rabbit polyclonal antibody (STJ11101106) at 1:500 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-Phospho-AKT1/AKT2/AKT3-S473/S474/S472 antibody (STJ11101106)

SKU:
STJ11101106

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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AKT3
AKT1
10000
207
208
AKT3_HUMAN
AKT1_HUMAN
AKT2_HUMAN
Specificity: A synthetic phosphorylated peptide around S473 of human AKT1/AKT2/AKT3AKT1 (NP_005154.2).
Immunogen Sequence: QFSYS
Tissue Specificity Q9Y243 - In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
P31749 - Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
P31751 - Widely expressed. Expressed in myoblasts.
Q9Y243 - In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
P31749 - Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
P31751 - Widely expressed. Expressed in myoblasts.
Alternative Antibody Names Anti-Rac-Gamma Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase Akt-3 antibody
Anti-Protein Kinase B Gamma antibody
Anti-Pkb Gamma antibody
Anti-Rac-Pk-Gamma antibody
Anti-Stk-2 antibody
Anti-AKT3 antibody
Anti-PKBG antibody
Anti-Rac-Alpha Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase B antibody
Anti-Pkb antibody
Anti-Protein Kinase B Alpha antibody
Anti-Pkb Alpha antibody
Anti-Proto-Oncogene C-Akt antibody
Anti-Rac-Pk-Alpha antibody
Anti-AKT1 antibody
Anti-PKB antibody
Anti-RAC antibody
Anti-Rac-Beta Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase Akt-2 antibody
Anti-Protein Kinase B Beta antibody
Anti-Pkb Beta antibody
Anti-Rac Protein Kinase Beta antibody
Anti-Rac-Pk-Beta antibody
Anti-AKT2 antibody
Anti-Rac-Gamma Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase Akt-3 antibody
Anti-Protein Kinase B Gamma antibody
Anti-Pkb Gamma antibody
Anti-Rac-Pk-Gamma antibody
Anti-Stk-2 antibody
Anti-AKT3 antibody
Anti-PKBG antibody
Anti-Rac-Alpha Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase B antibody
Anti-Pkb antibody
Anti-Protein Kinase B Alpha antibody
Anti-Pkb Alpha antibody
Anti-Proto-Oncogene C-Akt antibody
Anti-Rac-Pk-Alpha antibody
Anti-AKT1 antibody
Anti-PKB antibody
Anti-RAC antibody
Anti-Rac-Beta Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase Akt-2 antibody
Anti-Protein Kinase B Beta antibody
Anti-Pkb Beta antibody
Anti-Rac Protein Kinase Beta antibody
Anti-Rac-Pk-Beta antibody
Anti-AKT2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance