• Western blot analysis of extracts of various cell lines, using Phospho-Akt-S473 antibody (STJ11103745) at 1:1000 dilution. Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Akt-S473 Rabbit monoclonal antibody (STJ11103745) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Akt-S473 antibody (STJ11103745) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates using Phospho-Akt-S473 Rabbit monoclonal antibody (STJ11103745) at 1:1000 dilution. Both Jurkat and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-AKT1 & AKT2 & AKT3-S473 & S474 & S472 antibody [S5MR] (STJ11103745)

SKU:
STJ11103745

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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AKT2
AKT1
208
207
10000
AKT2_HUMAN
AKT1_HUMAN
AKT3_HUMAN
Specificity: A synthetic phosphorylated peptide around S473 of human AKT1 (NP_005154.2).
Immunogen Sequence: QFSYS
Tissue Specificity P31751 - Widely expressed. Expressed in myoblasts.
P31749 - Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
Q9Y243 - In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
P31751 - Widely expressed. Expressed in myoblasts.
P31749 - Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
Q9Y243 - In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
Alternative Antibody Names Anti-Rac-Beta Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase Akt-2 antibody
Anti-Protein Kinase B Beta antibody
Anti-Pkb Beta antibody
Anti-Rac Protein Kinase Beta antibody
Anti-Rac-Pk-Beta antibody
Anti-AKT2 antibody
Anti-Rac-Alpha Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase B antibody
Anti-Pkb antibody
Anti-Protein Kinase B Alpha antibody
Anti-Pkb Alpha antibody
Anti-Proto-Oncogene C-Akt antibody
Anti-Rac-Pk-Alpha antibody
Anti-AKT1 antibody
Anti-PKB antibody
Anti-RAC antibody
Anti-Rac-Gamma Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase Akt-3 antibody
Anti-Protein Kinase B Gamma antibody
Anti-Pkb Gamma antibody
Anti-Rac-Pk-Gamma antibody
Anti-Stk-2 antibody
Anti-AKT3 antibody
Anti-PKBG antibody
Anti-Rac-Beta Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase Akt-2 antibody
Anti-Protein Kinase B Beta antibody
Anti-Pkb Beta antibody
Anti-Rac Protein Kinase Beta antibody
Anti-Rac-Pk-Beta antibody
Anti-AKT2 antibody
Anti-Rac-Alpha Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase B antibody
Anti-Pkb antibody
Anti-Protein Kinase B Alpha antibody
Anti-Pkb Alpha antibody
Anti-Proto-Oncogene C-Akt antibody
Anti-Rac-Pk-Alpha antibody
Anti-AKT1 antibody
Anti-PKB antibody
Anti-RAC antibody
Anti-Rac-Gamma Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase Akt-3 antibody
Anti-Protein Kinase B Gamma antibody
Anti-Pkb Gamma antibody
Anti-Rac-Pk-Gamma antibody
Anti-Stk-2 antibody
Anti-AKT3 antibody
Anti-PKBG antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance