• Immunofluorescence analysis of HeLa cells using Phospho-Akt-Y315 Rabbit polyclonal antibody (STJ11105776) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Phospho-Akt-Y315 in paraffin-embedded rat brain using Phospho-Akt-Y315 Rabbit polyclonal antibody (STJ11105776) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Phospho-Akt-Y315 in paraffin-embedded mouse brain using Phospho-Akt-Y315 Rabbit polyclonal antibody (STJ11105776) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Phospho-Akt-Y315 in paraffin-embedded human brain using Phospho-Akt-Y315 Rabbit polyclonal antibody (STJ11105776) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-Akt-Y315 antibody (STJ11105776)

SKU:
STJ11105776

Current Stock:
Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:100
IF/ICC:1:100-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AKT1
AKT2
207
208
10000
AKT1_HUMAN
AKT2_HUMAN
AKT3_HUMAN
Specificity: A synthetic phosphorylated peptide around Y315 of human AKT1 (NP_001014431.1).
Immunogen Sequence: PEYLA
Tissue Specificity P31749 - Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
P31751 - Widely expressed. Expressed in myoblasts.
Q9Y243 - In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
P31749 - Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.
P31751 - Widely expressed. Expressed in myoblasts.
Q9Y243 - In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney.
Alternative Antibody Names Anti-Rac-Alpha Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase B antibody
Anti-Pkb antibody
Anti-Protein Kinase B Alpha antibody
Anti-Pkb Alpha antibody
Anti-Proto-Oncogene C-Akt antibody
Anti-Rac-Pk-Alpha antibody
Anti-AKT1 antibody
Anti-PKB antibody
Anti-RAC antibody
Anti-Rac-Beta Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase Akt-2 antibody
Anti-Protein Kinase B Beta antibody
Anti-Pkb Beta antibody
Anti-Rac Protein Kinase Beta antibody
Anti-Rac-Pk-Beta antibody
Anti-AKT2 antibody
Anti-Rac-Gamma Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase Akt-3 antibody
Anti-Protein Kinase B Gamma antibody
Anti-Pkb Gamma antibody
Anti-Rac-Pk-Gamma antibody
Anti-Stk-2 antibody
Anti-AKT3 antibody
Anti-PKBG antibody
Anti-Rac-Alpha Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase B antibody
Anti-Pkb antibody
Anti-Protein Kinase B Alpha antibody
Anti-Pkb Alpha antibody
Anti-Proto-Oncogene C-Akt antibody
Anti-Rac-Pk-Alpha antibody
Anti-AKT1 antibody
Anti-PKB antibody
Anti-RAC antibody
Anti-Rac-Beta Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase Akt-2 antibody
Anti-Protein Kinase B Beta antibody
Anti-Pkb Beta antibody
Anti-Rac Protein Kinase Beta antibody
Anti-Rac-Pk-Beta antibody
Anti-AKT2 antibody
Anti-Rac-Gamma Serine/Threonine-Protein Kinase antibody
Anti-Protein Kinase Akt-3 antibody
Anti-Protein Kinase B Gamma antibody
Anti-Pkb Gamma antibody
Anti-Rac-Pk-Gamma antibody
Anti-Stk-2 antibody
Anti-AKT3 antibody
Anti-PKBG antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance