Anti-Phospho-ACAP1-Ser554 antibody (520-569 aa) (STJ91177)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Centaurin-beta1 around the phosphorylation site of Ser554 at the amino acid range 520-569
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1-Ser554 (520-569 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000WB 1:500-2000IHC 1:100-1:300ELISA 1:10000-40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ACAP1 |
| Gene ID | 9744 |
| Uniprot ID | ACAP1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Centaurin-beta1 around the phosphorylation site of Ser554 at the amino acid range 520-569 |
| Immunogen Region | 520-569 aa |
| Specificity | Phospho-ACAP1 (S554) Polyclonal Antibody detects endogenous levels of ACAP1 protein only when phosphorylated at S554. |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration. |
| Function | GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration. |
| Protein Name | Arf-Gap With Coiled-Coil - Ank Repeat And Ph Domain-Containing Protein 1Centaurin-Beta-1Cnt-B1 |
| Database Links | |
| Cellular Localisation | Recycling Endosome MembranePeripheral Membrane ProteinCytoplasmic Side |
| Alternative Antibody Names | Anti-Arf-Gap With Coiled-Coil - Ank Repeat And Ph Domain-Containing Protein 1 antibodyAnti-Centaurin-Beta-1 antibodyAnti-Cnt-B1 antibodyAnti-ACAP1 antibodyAnti-CENTB1 antibodyAnti-KIAA0050 antibody |
Information sourced from Uniprot.org