• Immunofluorescence analysis of A549 cells using PHF11 antibody (STJ115387).
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using PHF11 Rabbit polyclonal antibody (STJ115387) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Mouse brain, using PHF11 antibody (STJ115387) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-PHF11 antibody (165-319) (STJ115387)

SKU:
STJ115387

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PHF11 (165-319) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PHF11
Gene ID: 51131
Uniprot ID: PHF11_HUMAN
Immunogen Region: 165-319
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 165-319 of human PHF11 (NP_001035533.1).
Immunogen Sequence: IAQSAKFSGVKRKRGRKKPL SGNHVQPPETMKCNTFIRQV KEEHGRHTDATVKVPFLKKC KEAGLLNYLLEEILDKVHSI PEKLMDETTSESDYEEIGSA LFDCRLFEDTFVNFQAAIEK KIHASQQRWQQLKEEIELLQ DLKQTLCSFQENRDL
Tissue Specificity Highly expressed in T and B-cells, as well as natural killer and mature dendritic cells. Expressed at higher levels in Th1 as compared to Th2 cells. Expressed at low levels in all normal tissues tested, including lung, testis, small intestine, breast, liver and placenta.
Function Positive regulator of Th1-type cytokine gene expression.
Protein Name Phd Finger Protein 11
Brca1 C-Terminus-Associated Protein
Renal Carcinoma Antigen Ny-Ren-34
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Phd Finger Protein 11 antibody
Anti-Brca1 C-Terminus-Associated Protein antibody
Anti-Renal Carcinoma Antigen Ny-Ren-34 antibody
Anti-PHF11 antibody
Anti-BCAP antibody

Information sourced from Uniprot.org

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