• Western blot analysis of various lysates using PHF11 Rabbit polyclonal antibody (STJ110113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-PHF11 antibody (1-292) (STJ110113)

SKU:
STJ110113

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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PHF11
Gene ID: 51131
Uniprot ID: PHF11_HUMAN
Immunogen Region: 1-292
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human PHF11 (NP_001035533.1).
Immunogen Sequence: MAQASPPRPERVLGASSPEA RPAQEALLLPTGVFQVAEKM EKRTCALCPKDVEYNVLYFA QSENIAAHENCLLYSSGLVE CEDQDPLNPDRSFDVESVKK EIQRGRKLKCKFCHKRGATV GCDLKNCNKNYHFFCAKKDD AVPQSDGVRGIYKLLCQQHA QFPIIAQSAKFSGVKRKRGR KKPLSGNHVQPPETMKCNTF IRQVKEEHGRHTDATVKVPF LKKCKEAGLLNYLLEEILD
Tissue Specificity Highly expressed in T and B-cells, as well as natural killer and mature dendritic cells. Expressed at higher levels in Th1 as compared to Th2 cells. Expressed at low levels in all normal tissues tested, including lung, testis, small intestine, breast, liver and placenta.
Function Positive regulator of Th1-type cytokine gene expression.
Protein Name Phd Finger Protein 11
Brca1 C-Terminus-Associated Protein
Renal Carcinoma Antigen Ny-Ren-34
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Phd Finger Protein 11 antibody
Anti-Brca1 C-Terminus-Associated Protein antibody
Anti-Renal Carcinoma Antigen Ny-Ren-34 antibody
Anti-PHF11 antibody
Anti-BCAP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance