• Western blot analysis of PGP9. 5. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:3000, RT, 1h.
  • Immunohistochemistry analysis of PGP9. 5. Sample: Human esophageal cancer, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, 98C, 20 min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • Immunohistochemistry analysis of PGP9. 5. Sample: Human stomach, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, 98C, 20 min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • Immunohistochemistry analysis of PGP9. 5. Sample: mouse brain, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, 98C, 20 min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • Immunohistochemistry analysis of PGP9. 5. Sample: mouse colon, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, 98C, 20 min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • Immunohistochemistry analysis of PGP9. 5. Sample: Rat brain, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, 98C, 20 min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • Immunohistochemistry analysis of PGP9. 5. Sample: Rat colon, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, 98C, 20 min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.
  • Immunohistochemistry analysis of PGP9. 5. Sample: Rat stomach, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, 98C, 20 min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.

Anti-PGP9.5 antibody [SM5228] (STJA0005228)

SKU:
STJA0005228

£170.50 - £233.50
Shipping:
Free Shipping
Current Stock:
Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-PGP9.5 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: SM5228
Conjugation: Unconjugated
Isotype: IgG1
Formulation: PBS with 0.02% sodium azide, 100 Mu g/ml BSA and 50% glycerol.
Purification: Affinity purification
Dilution Range: WB (H, M, R) 1:1000-1:2000
IHC/IF (H, M, R) 1:1000-1:2000
Storage Instruction: Store at-20C for up to one year, and avoid repeated freeze-thaw cycles.
Gene Symbol: Uchl1
Gene ID: 22223
Uniprot ID: UCHL1_MOUSE
Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse PGP9.5
Post Translational Modifications O-glycosylated.
Function Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis. Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1. In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways. Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production. Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes. Mediates the 'Lys-48'-linked deubiquitination of the transcriptional coactivator WWTR1/TAZ leading to its stabilization and inhibition of osteoclastogenesis. Deubiquitinates and stabilizes epidermal growth factor receptor EGFR to prevent its degradation and to activate its downstream mediators. Modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins. Enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by abrogateing its VHL E3 ligase-mediated ubiquitination and consequently inhibiting its degradation.
Protein Name Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1
Uch-L1
Neuron Cytoplasmic Protein 9.5
Pgp 9.5
Pgp9.5
Ubiquitin Thioesterase L1
Database Links Reactome: R-MMU-5689603
Cellular Localisation Cytoplasm
Endoplasmic Reticulum Membrane
Lipid-Anchor
Localizes Near And/Or Within Mitochondria To Potentially Interact With Mitochondrial Proteins
Alternative Antibody Names Anti-Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1 antibody
Anti-Uch-L1 antibody
Anti-Neuron Cytoplasmic Protein 9.5 antibody
Anti-Pgp 9.5 antibody
Anti-Pgp9.5 antibody
Anti-Ubiquitin Thioesterase L1 antibody
Anti-Uchl1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance