• Western blot analysis of extracts of various cell lines, using PLGF antibody (STJ24971) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.
  • Western blot analysis of extracts of various cell lines, using PLGF antibody (STJ24971) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Anti-PGF antibody (19-170) (STJ24971)

SKU:
STJ24971

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PGF
Gene ID: 5228
Uniprot ID: PLGF_HUMAN
Immunogen Region: 19-170
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 19-170 of human PLGF (NP_002623.2).
Immunogen Sequence: LPAVPPQQWALSAGNGSSEV EVVPFQEVWGRSYCRALERL VDVVSEYPSEVEHMFSPSCV SLLRCTGCCGDENLHCVPVE TANVTMQLLKIRSGDRPSYV ELTFSQHVRCECRPLREKMK PERRRPKGRGKRRREKQRPT DCHLCGDAVPRR
Tissue Specificity While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
Post Translational Modifications N-glycosylated.
Function Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.
Protein Name Placenta Growth Factor
Plgf
Database Links Reactome: R-HSA-194313
Reactome: R-HSA-195399
Cellular Localisation Secreted
The Three Isoforms Are Secreted But Plgf-2 Appears To Remain Cell Attached Unless Released By Heparin
Alternative Antibody Names Anti-Placenta Growth Factor antibody
Anti-Plgf antibody
Anti-PGF antibody
Anti-PGFL antibody
Anti-PLGF antibody

Information sourced from Uniprot.org

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