• Immunohistochemistry analysis of paraffin-embedded human stomach using Pepsinogen II (PG II) Rabbit monoclonal antibody (STJ11104788) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Pepsinogen II (PG II) Rabbit monoclonal antibody (STJ11104788) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PGC antibody (1-388) [S4788RM] (STJ11104788)

SKU:
STJ11104788

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Gastricsin (1-388) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4788RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PGC
Gene ID: 5225
Uniprot ID: PEPC_HUMAN
Immunogen Region: 1-388
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-388 of human Pepsinogen II (PG II) (NP_002621.1).
Immunogen Sequence: MKWMVVVLVCLQLLEAAVVK VPLKKFKSIRETMKEKGLLG EFLRTHKYDPAWKYRFGDLS VTYEPMAYMDAAYFGEISIG TPPQNFLVLFDTGSSNLWVP SVYCQSQACTSHSRFNPSES STYSTNGQTFSLQYGSGSLT GFFGYDTLTVQSIQVPNQEF GLSENEPGTNFVYAQFDGIM GLAYPALSVDEATTAMQGMV QEGALTSPVFSVYLSNQQGS SGGAVVFGGVDSSLYTGQI
Function Hydrolyzes a variety of proteins.
Protein Name Gastricsin
Pepsinogen C
Cellular Localisation Secreted
Alternative Antibody Names Anti-Gastricsin antibody
Anti-Pepsinogen C antibody
Anti-PGC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance