• Immunofluorescence analysis of L929 cells using PGAM1 Rabbit polyclonal antibody (STJ24968) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using PGAM1 antibody (STJ24968) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PGAM1 antibody (1-254) (STJ24968)

SKU:
STJ24968

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PGAM1 (1-254) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PGAM1
Gene ID: 5223
Uniprot ID: PGAM1_HUMAN
Immunogen Region: 1-254
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-254 of human PGAM1 (NP_002620.1).
Immunogen Sequence: MAAYKLVLIRHGESAWNLEN RFSGWYDADLSPAGHEEAKR GGQALRDAGYEFDICFTSVQ KRAIRTLWTVLDAIDQMWLP VVRTWRLNERHYGGLTGLNK AETAAKHGEAQVKIWRRSYD VPPPPMEPDHPFYSNISKDR RYADLTEDQLPSCESLKDTI ARALPFWNEEIVPQIKEGKR VLIAAHGNSLRGIVKHLEGL SEEAIMELNLPTGIPIVYEL DKNLKPIKPMQFLGDEETV
Tissue Specificity Expressed in the liver and brain. Not found in the muscle.
Post Translational Modifications Acetylated at Lys-253, Lys-253 and Lys-254 under high glucose condition. Acetylation increases catalytic activity. Under glucose restriction SIRT1 levels dramatically increase and it deacetylates the enzyme.
Function Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglyceratea crucial step in glycolysis, by using 2,3-bisphosphoglycerate. Also catalyzes the interconversion of (2R)-2,3-bisphosphoglycerate and (2R)-3-phospho-glyceroyl phosphate.
Protein Name Phosphoglycerate Mutase 1
Bpg-Dependent Pgam 1
Phosphoglycerate Mutase Isozyme B
Pgam-B
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-70171
Reactome: R-HSA-70263
Alternative Antibody Names Anti-Phosphoglycerate Mutase 1 antibody
Anti-Bpg-Dependent Pgam 1 antibody
Anti-Phosphoglycerate Mutase Isozyme B antibody
Anti-Pgam-B antibody
Anti-PGAM1 antibody
Anti-PGAMA antibody
Anti-CDABP0006 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance