• Immunofluorescence analysis of U-2 OS cells using PFDN4 Rabbit polyclonal antibody (STJ117495) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using PFDN4 Rabbit polyclonal antibody (STJ117495) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PFDN4 Rabbit polyclonal antibody (STJ117495) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PFDN4 Rabbit polyclonal antibody (STJ117495) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PFDN4 antibody (1-134) (STJ117495)

SKU:
STJ117495

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PFDN4 (1-134) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PFDN4
Gene ID: 5203
Uniprot ID: PFD4_HUMAN
Immunogen Region: 1-134
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-134 of human PFDN4 (NP_002614.2).
Immunogen Sequence: MAATMKKAAAEDVNVTFEDQ QKINKFARNTSRITELKEEI EVKKKQLQNLEDACDDIMLA DDDCLMIPYQIGDVFISHSQ EETQEMLEEAKKNLQEEIDA LESRVESIQRVLADLKVQLY AKFGSNINLEADES
Function Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
Protein Name Prefoldin Subunit 4
Protein C-1
Database Links Reactome: R-HSA-389957
Cellular Localisation Nucleus
Cytoplasm
Mitochondrion
Alternative Antibody Names Anti-Prefoldin Subunit 4 antibody
Anti-Protein C-1 antibody
Anti-PFDN4 antibody
Anti-PFD4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance